ArticleThe Journal of biological chemistry2012
The organization of a CSN5-containing subcomplex of the COP9 signalosome.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Functional Characterization of OsCSN1 in the Agronomic Trait Control of Rice Seedlings Under Far-Red Light.International journal of molecular sciences · 2025Article
- Altered assembly paths mitigate interference among paralogous complexes.Nature communications · 2024Article
- Fungal COP9 signalosome assembly requires connection of two trimeric intermediates for integration of intrinsic deneddylase.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Desmosomal COP9 regulates proteome degradation in arrhythmogenic right ventricular dysplasia/cardiomyopathy.The Journal of clinical investigation · 2021Article
- CSN6: a promising target for cancer prevention and therapy.Histology and histopathology · 2020Review
- CSN6: a promising target for cancer prevention and therapy.Histology and histopathology · 2020Review
- Deubiquitinating Enzyme: A Potential Secondary Checkpoint of Cancer Immunity.Frontiers in oncology · 2020Review
- Role of Cop9 Signalosome Subunits in the Environmental and Hormonal Balance of Plant.Biomolecules · 2019Review
- Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis.Cell cycle (Georgetown, Tex.) · 2019Article
- COP9 Signalosome CSN4 and CSN5 Subunits Are Involved in Jasmonate-Dependent Defense Against Root-Knot Nematode in Tomato.Frontiers in plant science · 2019Article
- COP9 signalosome subunit CSN5, but not CSN6, is upregulated in lung adenocarcinoma and predicts poor prognosis.Journal of thoracic disease · 2018Article
- The Arabidopsis Cop9 signalosome subunit 4 (CNS4) is involved in adventitious root formation.Scientific reports · 2017Article
- Analysis of RNA expression of normal and cancer tissues reveals high correlation of COP9 gene expression with respiratory chain complex components.BMC genomics · 2016Article
- The DenA/DEN1 Interacting Phosphatase DipA Controls Septa Positioning and Phosphorylation-Dependent Stability of Cytoplasmatic DenA/DEN1 during Fungal Development.PLoS genetics · 2016Article
- Tackling the crystallographic structure determination of the COP9 signalosome.Acta crystallographica. Section D, Structural biology · 2016Article
- The devil is in the details: comparison between COP9 signalosome (CSN) and the LID of the 26S proteasome.Current genetics · 2016Review
- The Role of the COP9 Signalosome and Neddylation in DNA Damage Signaling and Repair.Biomolecules · 2015Review
- The CSN/COP9 signalosome regulates synaptonemal complex assembly during meiotic prophase I of Caenorhabditis elegans.PLoS genetics · 2014Article
- COP9 subunits 4 and 5 target soluble guanylyl cyclase α1 and p53 in prostate cancer cells.Molecular endocrinology (Baltimore, Md.) · 2014Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The COP9 signalosome (CSN) is an evolutionarily conserved multi-protein complex that interfaces with the ubiquitin-proteasome pathway and plays critical developmental roles in both animals and plants. Although some subunits are present only in an ∼320-kDa complex-dependent form, other subunits are also detected in configurations distinct from the 8-subunit holocomplex. To date, the only known biochemical activity intrinsic to the complex, deneddylation of the Cullin subunits from Cullin-RING ubiquitin ligases, is assigned to CSN5. As an essential step to understanding the structure and assembly of a CSN5-containing subcomplex of the CSN, we reconstituted a CSN4-5-6-7 subcomplex. The core of the subcomplex is based on a stable heterotrimeric association of CSN7, CSN4, and CSN6, requiring coexpression in a bacterial reconstitution system. To this heterotrimer, we could then add CSN5 in vitro to reconstitute a quaternary complex. Using biochemical and biophysical methods, we identified pairwise and combinatorial interactions necessary for the formation of the CSN4-5-6-7 subcomplex. The subcomplex is stabilized by three types of interactions: MPN-MPN between CSN5 and CSN6, PCI-PCI between CSN4 and CSN7, and interactions mediated through the CSN6 C terminus with CSN4 and CSN7. CSN8 was also found to interact with the CSN4-6-7 core. These data provide a strong framework for further investigation of the organization and assembly of this pivotal regulatory complex.
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