ArticleApplied microbiology and biotechnology2013
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
Article in Applied microbiology and biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
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- Duplex real-time PCR assay with high-resolution melt analysis for the detection and quantification ofJournal of food science and technology · 2023Article
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- p53 Hinders CRISPR/Cas9-Mediated Targeted Gene Disruption in Memory CD8 T Cells In Vivo.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
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- Development of a real-time PCR method for the genoserotyping of Salmonella Paratyphi B variant Java.Applied microbiology and biotechnology · 2019Article
- Detection and discrimination of five E. coli pathotypes using a combinatory SYBR® Green qPCR screening system.Applied microbiology and biotechnology · 2018Article
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- A molecular approach for the rapid, selective and sensitive detection of Exophiala jeanselmei in environmental samples: development and performance assessment of a real-time PCR assay.Applied microbiology and biotechnology · 2016Article
- Mining of novel species-specific primers for PCR detection of Listeria monocytogenes based on genomic approach.World journal of microbiology & biotechnology · 2015Article
- Use of next generation sequencing data to develop a qPCR method for specific detection of EU-unauthorized genetically modified Bacillus subtilis overproducing riboflavin.BMC biotechnology · 2015Article
- Development and performance assessment of a qualitative SYBR® green real-time PCR assay for the detection of Aspergillus versicolor in indoor air.Applied microbiology and biotechnology · 2015Article
- A new rapid method for detecting epidermal growth factor receptor mutations in non-small cell lung cancer.Oncology reports · 2015Article
- An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food.Frontiers in microbiology · 2015Review
- SYBR®Green qPCR Salmonella detection system allowing discrimination at the genus, species and subspecies levels.Applied microbiology and biotechnology · 2013Article
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5 authors.
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Abstract
A combination of four qualitative SYBR®Green qPCR screening assays targeting two levels of discrimination: Listeria genus (except Listeria grayi) and Listeria monocytogenes, is presented. These assays have been developed to be run simultaneously using the same polymerase chain reaction (PCR) programme. The paper also proposes a new validation procedure to specifically validate qPCR assays applied to food microbiology according to two guidelines: the ISO 22118 norm and the "Definition of minimum performance requirements for analytical methods of GMO testing". The developed assays target the iap, prs and hlyA genes that belong to or neighbour the virulence cluster of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the SYBR®Green qPCR assays is two to five copies of target genes per qPCR reaction. These assays are highly accurate (98.08 and 100 % accuracy for the Listeria spp. and L. monocytogenes assays, respectively).
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