ArticleNature biotechnology2012
Engineering a thermoregulated intein-modified xylanase into maize for consolidated lignocellulosic biomass processing.
Article in Nature biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 86 citations in OpenAlex.
- Engineering microalgal cell wall-anchored proteins using GP1 PPSPX motifs and releasing with intein-mediated fusion.bioRxiv : the preprint server for biology · 2025Article
- Thermally controlled intein splicing of engineered DNA polymerases provides a robust and generalizable solution for accurate and sensitive molecular diagnostics.Nucleic acids research · 2023Article
- Genetic engineering of complex feed enzymes into barley seed for direct utilization in animal feedstuff.Plant biotechnology journal · 2023Article
- Iron incorporation both intra- and extra-cellularly improves the yield and saccharification of switchgrass (Panicum virgatum L.) biomass.Biotechnology for biofuels · 2021Article
- Construction and Quantitation of a Selectable Protein Splicing Sensor Using Gibson Assembly and Spot Titers.Current protocols · 2021Article
- Inteins in Science: Evolution to Application.Microorganisms · 2020Review
- Transgene Biocontainment Strategies for Molecular Farming.Frontiers in plant science · 2020Review
- Use of the lignocellulose-degrading bacteriumBiotechnology for biofuels · 2020Article
- Polymer conjugation of proteins as a synthetic post-translational modification to impact their stability and activity.Polymer chemistry · 2019Article
- Biotechnological Applications of Protein Splicing.Current protein & peptide science · 2019Review
- Tunnel engineering to accelerate product release for better biomass-degrading abilities in lignocellulolytic enzymes.Biotechnology for biofuels · 2019Article
- Exploring the Treasure of Plant Molecules With Integrated Biorefineries.Frontiers in plant science · 2019Article
- Xylan in the Middle: Understanding Xylan Biosynthesis and Its Metabolic Dependencies Toward Improving Wood Fiber for Industrial Processing.Frontiers in plant science · 2019Review
- Peptide backbone circularization enhances antifreeze protein thermostability.Protein science : a publication of the Protein Society · 2017Article
- In planta expression of hyperthermophilic enzymes as a strategy for accelerated lignocellulosic digestion.Scientific reports · 2017Article
- Genetic engineering of grass cell wall polysaccharides for biorefining.Plant biotechnology journal · 2017Review
- Transcriptome analysis of genes involved in secondary cell wall biosynthesis in developing internodes of Miscanthus lutarioriparius.Scientific reports · 2017Article
- Towards an Understanding of Enhanced Biomass Digestibility by In Planta Expression of a Family 5 Glycoside Hydrolase.Scientific reports · 2017Article
- In planta production and characterization of a hyperthermostable GH10 xylanase in transgenic sugarcane.Plant molecular biology · 2017Article
- Strategies for the production of cell wall-deconstructing enzymes in lignocellulosic biomass and their utilization for biofuel production.Plant biotechnology journal · 2016Review
Corrections and comments
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Authors and funding
17 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant cellulosic biomass is an abundant, low-cost feedstock for producing biofuels and chemicals. Expressing cell wall-degrading (CWD) enzymes (e.g. xylanases) in plant feedstocks could reduce the amount of enzymes required for feedstock pretreatment and hydrolysis during bioprocessing to release soluble sugars. However, in planta expression of xylanases can reduce biomass yield and plant fertility. To overcome this problem, we engineered a thermostable xylanase (XynB) with a thermostable self-splicing bacterial intein to control the xylanase activity. Intein-modified XynB (iXynB) variants were selected that have <10% wild-type enzymatic activity but recover >60% enzymatic activity upon intein self-splicing at temperatures >59 °C. Greenhouse-grown xynB maize expressing XynB has shriveled seeds and low fertility, but ixynB maize had normal seeds and fertility. Processing dried ixynB maize stover by temperature-regulated xylanase activation and hydrolysis in a cocktail of commercial CWD enzymes produced >90% theoretical glucose and >63% theoretical xylose yields.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.