ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2013
Actinin-4 in keratinocytes regulates motility via an effect on lamellipodia stability and matrix adhesions.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Placental proteome in late‑onset of fetal growth restriction.Molecular medicine reports · 2022Article
- Wound Healing from an Actin Cytoskeletal Perspective.Cold Spring Harbor perspectives in biology · 2022Review
- Alpha-actinin-4 is essential for maintaining normal trophoblast proliferation and differentiation during early pregnancy.Reproductive biology and endocrinology : RB&E · 2021Article
- ACTN4 Promotes the Proliferation, Migration, Metastasis of Osteosarcoma and Enhances its Invasive Ability through the NF-κB Pathway.Pathology oncology research : POR · 2020Article
- Differential expression profiles of long non‑coding RNAs during the mouse pronuclear stage under normal gravity and simulated microgravity.Molecular medicine reports · 2019Article
- Tyro3-mediated phosphorylation of ACTN4 at tyrosines is FAK-dependent and decreases susceptibility to cleavage by m-Calpain.The international journal of biochemistry & cell biology · 2018Article
- α-Actinin-4 promotes metastasis in gastric cancer.Laboratory investigation; a journal of technical methods and pathology · 2017Article
- A hemidesmosomal protein regulates actin dynamics and traction forces in motile keratinocytes.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Alpha actinin-1 regulates cell-matrix adhesion organization in keratinocytes: consequences for skin cell motility.The Journal of investigative dermatology · 2015Article
- Integrins in Wound Healing.Advances in wound care · 2014Review
- α-Actinin-4 is required for amoeboid-type invasiveness of melanoma cells.The Journal of biological chemistry · 2014Article
- Ion channels and transporters in tumour cell migration and invasion.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Focal Contact and Hemidesmosomal Proteins in Keratinocyte Migration and Wound Repair.Advances in wound care · 2014Review
- Aggregation state determines the localization and function of M1- and M23-aquaporin-4 in astrocytes.The Journal of cell biology · 2014Article
- Focal adhesion complex proteins in epidermis and squamous cell carcinoma.Cell cycle (Georgetown, Tex.) · 2013Review
- The switch-associated protein 70 (SWAP-70) bundles actin filaments and contributes to the regulation of F-actin dynamics.The Journal of biological chemistry · 2013Article
- Networking galore: intermediate filaments and cell migration.Current opinion in cell biology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
During wound repair, epidermal cells at the edge of an injury establish front-rear polarity through orchestrated changes in their cytoskeleton and adhesion structures. The polarity and directed migration of such cells is determined by the assembly, extension, and stabilization of a lamellipodium. Actinin-4 associates with lamellipodia and has been implicated in regulating lamellipodial structure, function and assembly. To study the functions of actinin-4 in human keratinocytes, we used shRNA to generate knockdown cells and compared their motility behavior and matrix adhesion assembly to scrambled shRNA treated control keratinocytes. Actinin-4 knockdown keratinocytes lack polarity, assemble multiple lamellipodia with a 2× increased area over controls, display reduced activity of the actin remodeling protein cofilin, and fail to migrate in a directional manner. This motility defect is rescued by plating knockdown cells on preformed laminin-332 matrix. In actinin-4-knockdown keratinocytes, focal contact area is increased by 25%, and hemidesmosome proteins are mislocalized. Specifically, α6β4 integrin localizes to large lamellipodial extensions, displays reduced dynamics, and fails to recruit its bullous pemphigoid antigen binding partners. Together, our data indicate a role for actinin-4 in regulating the steering mechanism of keratinocytes via profound effects on their matrix adhesion sites.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.