ArticleJournal of biomedical optics2012
Impact of the optical depth of field on cytogenetic image quality.
Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Label-Free Quantification of Protein Density in Living Cells.Current protocols · 2025Article
- Development and Assessment of Multiple Illumination Color Fourier Ptychographic Microscopy for High Throughput Sample Digitization.Sensors (Basel, Switzerland) · 2024Article
- Using symmetric illumination and color camera to achieve high throughput Fourier ptychographic microscopy.Journal of biophotonics · 2023Article
- Using Fourier ptychography microscopy to achieve high-resolution chromosome imaging: an initial evaluation.Journal of biomedical optics · 2022Article
- Imaging live cells at the nanometer-scale with single-molecule microscopy: obstacles and achievements in experiment optimization for microbiology.Molecules (Basel, Switzerland) · 2014Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In digital pathology, clinical specimen slides are converted into digital images by microscopic image scanners. Since random vibration and mechanical drifting are unavoidable on even high-precision moving stages, the optical depth of field (DOF) of microscopic systems may affect image quality, in particular when using an objective lens with high magnification power. The DOF of a microscopic system was theoretically analyzed and experimentally validated using standard resolution targets under 60× dry and 100× oil objective lenses, respectively. Then cytogenetic samples were imaged at in-focused and off-focused states to analyze the impact of DOF on the acquired image qualities. For the investigated system equipped with the 60× dry and 100× oil objective lenses, the theoretical estimation of the DOF are 0.855 μm and 0.703 μm, and the measured DOF are 3.0 μm and 1.8 μm, respectively. The observation reveals that the chromosomal bands of metaphase cells are distinguishable when images are acquired up to approximately 1.5 μm or 1 μm out of focus using the 60× dry and 100× oil objective lenses, respectively. The results of this investigation provide important designing trade-off parameters to optimize the digital microscopic image scanning systems in the future.
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Registered trials
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