ArticleJournal of biomedical optics2012
Optical mapping at increased illumination intensities.
Article in Journal of biomedical optics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- A Multi-Task Deep Learning Framework for Characterizing Beating Behavior and Synchrony in Cardiomyocyte Clusters.Bioengineering (Basel, Switzerland) · 2026Article
- KairoSight-3.0: A validated optical mapping software to characterize cardiac electrophysiology, excitation-contraction coupling, and alternans.Journal of molecular and cellular cardiology plus · 2023Article
- Living myocardial slices: Advancing arrhythmia research.Frontiers in physiology · 2023Review
- Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology.Journal of biomedical optics · 2023Article
- Article
- In vivo ratiometric optical mapping enables high-resolution cardiac electrophysiology in pig models.Cardiovascular research · 2019Article
- Cardiac Optogenetics and Optical Mapping - Overcoming Spectral Congestion in All-Optical Cardiac Electrophysiology.Frontiers in physiology · 2019Review
- Evaluation of excitation propagation in the rabbit heart: optical mapping and transmural microelectrode recordings.PloS one · 2015Article
- Evolution of action potential alternans in rabbit heart during acute regional ischemia.BioMed research international · 2015Article
- Toward microendoscopy-inspired cardiac optogenetics in vivo: technical overview and perspective.Journal of biomedical optics · 2014Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Voltage-sensitive fluorescent dyes have become a major tool in cardiac and neuro-electrophysiology. Achieving high signal-to-noise ratios requires increased illumination intensities, which may cause photobleaching and phototoxicity. The optimal range of illumination intensities varies for different dyes and must be evaluated individually. We evaluate two dyes: di-4-ANBDQBS (excitation 660 nm) and di-4-ANEPPS (excitation 532 nm) in the guinea pig heart. The light intensity varies from 0.1 to 5 mW/mm2, with the upper limit at 5 to 10 times above values reported in the literature. The duration of illumination was 60 s, which in guinea pigs corresponds to 300 beats at a normal heart rate. Within the identified duration and intensity range, neither dye shows significant photobleaching or detectable phototoxic effects. However, light absorption at higher intensities causes noticeable tissue heating, which affects the electrophysiological parameters. The most pronounced effect is a shortening of the action potential duration, which, in the case of 532-nm excitation, can reach ∼30%. At 660-nm excitation, the effect is ∼10%. These findings may have important implications for the design of optical mapping protocols in biomedical applications.
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Registered trials
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