ArticleMolecules (Basel, Switzerland)2012
MiR-15a decreases bovine mammary epithelial cell viability and lactation and regulates growth hormone receptor expression.
Article in Molecules (Basel, Switzerland), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- Differential Expression of MicroRNAs in the Mammary Gland of Lactating and Dry Murciano-Granadina Goats.Animals : an open access journal from MDPI · 2026Article
- Screening of mtr-miR156a from exosomes of dairy cow blood to milk and its regulatory effect on milk protein synthesis in BMECs.BMC genomics · 2024Article
- Comprehensive Profiling of ceRNA (circRNA-miRNA-mRNA) Networks in Hypothalamic-Pituitary-Mammary Gland Axis of Dairy Cows under Heat Stress.International journal of molecular sciences · 2023Article
- Article
- Integrated analysis of expression profiles with meat quality traits in cattle.Scientific reports · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Review
- Integrated Small RNA Sequencing, Transcriptome and GWAS Data Reveal microRNA Regulation in Response to Milk Protein Traits in Chinese Holstein Cattle.Frontiers in genetics · 2021Article
- circ-016910 sponges miR-574-5p to regulate cell physiology and milk synthesis via MAPK and PI3K/AKT-mTOR pathways in GMECs.Journal of cellular physiology · 2020Article
- Comprehensive MicroRNA Expression Profile of the Mammary Gland in Lactating Dairy Cows With Extremely Different Milk Protein and Fat Percentages.Frontiers in genetics · 2020Article
- MicroRNA-15a Regulates the Differentiation of Intramuscular Preadipocytes by TargetingInternational journal of molecular sciences · 2019Article
- MiR-24-3p regulates cell proliferation and milk protein synthesis of mammary epithelial cells through menin in dairy cows.Journal of cellular physiology · 2019Article
- Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs).BMC veterinary research · 2018Article
- Genome Wide Identification of Novel Long Non-coding RNAs and Their Potential Associations With Milk Proteins in Chinese Holstein Cows.Frontiers in genetics · 2018Article
- Co-Expression Network and Pathway Analyses Reveal Important Modules of miRNAs Regulating Milk Yield and Component Traits.International journal of molecular sciences · 2017Article
- MicroRNA roles in signalling during lactation: an insight from differential expression, time course and pathway analyses of deep sequence data.Scientific reports · 2017Article
- Sunflower oil supplementation affects the expression of miR-20a-5p and miR-142-5p in the lactating bovine mammary gland.PloS one · 2017Article
- Differential Expression of the Alpha S1 Casein and Beta-Lactoglobulin Genes in Different Physiological Stages of the Adani Goats Mammary Glands.Iranian journal of biotechnology · 2016Article
- Lactation-Related MicroRNA Expression in Microvesicles of Human Umbilical Cord Blood.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow.BMC veterinary research · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs that regulate the expression of target genes at the post-transcriptional level by transcript degradation or translational inhibition. The role of bta-miR-15a in bovine mammary gland hasn’t been reported. Using miRNAs prediction software, GHR gene was predicted to be a potential target of bta-miR-15a. In this study, bovine mammary epithelial cell line was used as an in vitro cell model to address the function of bta-miR-15a on bovine mammary epithelial cells. The expression changes of bta-miR-15a and Ghr after bta-miR-15a transfection were detected by qRT-PCR; the expression of GHR protein and casein was detected by western blotting. To determine whether bta-miR-15a can affect cell viability, cells were examined using an electronic Coulter counter (CASY-TT). In conclusion, bta-miR-15a inhibited the expression of casein of bovine mammary epithelial cells, and cell number and viability were reduced by bta-miR-15a expression. Bta-miR-15a inhibited the viability of mammary epithelial cells as well as the expression of GHR mRNA and protein level, therefore suggesting that bta-miR-15a may play an important role in mammary gland physiology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.