Evidence map›Paper›PMID 23085614›Full record

ArticleNature methods2012

Membrane-protein binding measured with solution-phase plasmonic nanocube sensors.

Hung-Jen Wu, Joel Henzie, Wan-Chen Lin, Christopher Rhodes, Zhu Li, Elodie Sartorel, Jeremy Thorner, Peidong Yang, Jay T Groves

Abstract read
In one paragraph

Article in Nature methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Hung-Jen WuHoward Hughes Medical Institute, University of California, Berkeley, California, USA.
Joel Henzie
Wan-Chen Lin
Christopher Rhodes
Zhu Li
Elodie Sartorel
Jeremy Thorner
Peidong Yang
Jay T Groves

Funding

Yeast Protein Kinase Signaling Networks: Specificity, Regulation and FunctionR01GM021841 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI THORNER, JEREMY W. · 1985 to 2019
$11.8M
YEAST PEPTIDE HORMONE SYNTHESIS AND SIGNAL TRANSDUCTIONR37GM021841 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI THORNER, JEREMY W. · 1989 to 1998
–
Howard Hughes Medical InstituteNIGMS NIH HHS GM21841NIGMS NIH HHS R01 GM021841
6 · The paper itself

Abstract

We describe a solution-phase sensor of lipid-protein binding based on localized surface plasmon resonance (LSPR) of silver nanocubes. When silica-coated nanocubes are mixed in a suspension of lipid vesicles, supported membranes spontaneously assemble on their surfaces. Using a standard laboratory spectrophotometer, we calibrated the LSPR peak shift due to protein binding to the membrane surface and then characterized the lipid-binding specificity of a pleckstrin homology domain protein.

Indexed as

Protein BindingCalibrationLipid BilayersMembrane ProteinsMetal NanoparticlesNanotechnologySilicon DioxideSilverSolutionsSpectrometry, FluorescenceSpectrophotometry, UltravioletSurface Plasmon ResonanceLipid BilayersMembrane ProteinsSilicon DioxideSilverSolutions

Identifiers

PMID23085614
PMCPMC3703907

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.