ArticleMethods in enzymology2012
Reassembly of functionally intact environmental DNA-derived biosynthetic gene clusters.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next-Generation Sequencing Approach.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Metathramycin, a new bioactive aureolic acid discovered by heterologous expression of a metagenome derived biosynthetic pathway.RSC chemical biology · 2021Article
- Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.Frontiers in microbiology · 2020Article
- Uncovering the biosynthetic potential of rare metagenomic DNA using co-occurrence network analysis of targeted sequences.Nature communications · 2019Article
- Cadasides, Calcium-Dependent Acidic Lipopeptides from the Soil Metagenome That Are Active against Multidrug-Resistant Bacteria.Journal of the American Chemical Society · 2019Article
- Atolypenes, Tricyclic Bacterial Sesterterpenes Discovered Using a Multiplexed In Vitro Cas9-TAR Gene Cluster Refactoring Approach.ACS synthetic biology · 2019Article
- High-Yield Production of Herbicidal Thaxtomins and Thaxtomin Analogs in a Nonpathogenic Streptomyces Strain.Applied and environmental microbiology · 2018Article
- Culture-independent discovery of the malacidins as calcium-dependent antibiotics with activity against multidrug-resistant Gram-positive pathogens.Nature microbiology · 2018Article
- Bacterial natural product biosynthetic domain composition in soil correlates with changes in latitude on a continent-wide scale.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016Review
- Direct Capture Technologies for Genomics-Guided Discovery of Natural Products.Current topics in medicinal chemistry · 2016Review
- Mapping Microbial Response Metabolomes for Induced Natural Product Discovery.ACS chemical biology · 2015Article
- Yeast homologous recombination-based promoter engineering for the activation of silent natural product biosynthetic gene clusters.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Directed natural product biosynthesis gene cluster capture and expression in the model bacterium Bacillus subtilis.Scientific reports · 2015Article
- Mining soil metagenomes to better understand the evolution of natural product structural diversity: pentangular polyphenols as a case study.Journal of the American Chemical Society · 2014Article
- Arixanthomycins A-C: Phylogeny-guided discovery of biologically active eDNA-derived pentangular polyphenols.ACS chemical biology · 2014Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Only a small fraction of the bacterial diversity present in natural microbial communities is regularly cultured in the laboratory. Those bacteria that remain recalcitrant to culturing cannot be examined for the production of bioactive secondary metabolites using standard pure-culture approaches. The screening of genomic DNA libraries containing DNA isolated directly from environmental samples (environmental DNA (eDNA)) provides an alternative approach for studying the biosynthetic capacities of these organisms. One drawback of this approach has been that most eDNA isolation procedures do not permit the cloning of DNA fragments of sufficient length to capture large natural product biosynthetic gene clusters in their entirety. Although the construction of eDNA libraries with inserts big enough to capture biosynthetic gene clusters larger than ∼40kb remains challenging, it is possible to access large gene clusters by reassembling them from sets of smaller overlapping fragments using transformation-associated recombination in Saccharomyces cerevisiae. Here, we outline a method for the reassembly of large biosynthetic gene clusters from captured sets of overlapping soil eDNA cosmid clones. Natural product biosynthetic gene clusters reassembled using this approach can then be used directly for functional heterologous expression studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.