ArticleMethods in enzymology2012
DNA assembler: a synthetic biology tool for characterizing and engineering natural product gene clusters.
Article in Methods in enzymology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Advancing microbiota therapeutics: the role of synthetic biology in engineering microbial communities for precision medicine.Frontiers in bioengineering and biotechnology · 2024Review
- Harnessing phosphonate antibiotics argolaphos biosynthesis enables a synthetic biology-based green synthesis of glyphosate.Nature communications · 2022Article
- Efficient and Reproducible Multigene Expression after Single-Step Transfection Using Improved BAC Transgenesis and Engineering Toolkit.ACS synthetic biology · 2020Article
- Genetic platforms for heterologous expression of microbial natural products.Natural product reports · 2019Review
- Heterologous expression-facilitated natural products' discovery in actinomycetes.Journal of industrial microbiology & biotechnology · 2019Review
- AFEAP cloning: a precise and efficient method for large DNA sequence assembly.BMC biotechnology · 2017Article
- Twin-primer non-enzymatic DNA assembly: an efficient and accurate multi-part DNA assembly method.Nucleic acids research · 2017Article
- Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units inBiotechnology for biofuels · 2016Article
- Mapping Microbial Response Metabolomes for Induced Natural Product Discovery.ACS chemical biology · 2015Article
- The re-emergence of natural products for drug discovery in the genomics era.Nature reviews. Drug discovery · 2015Review
- Recent advances in natural product discovery.Current opinion in biotechnology · 2014Review
- Strategies for mining fungal natural products.Journal of industrial microbiology & biotechnology · 2014Review
- DNA assembly techniques for next-generation combinatorial biosynthesis of natural products.Journal of industrial microbiology & biotechnology · 2014Review
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
The majority of existing antibacterial and anticancer drugs are natural products or their derivatives. However, the characterization and engineering of these compounds are often hampered by limited ability to manipulate the corresponding biosynthetic pathways. Recently, we developed a genomics-driven, synthetic biology-based method, DNA assembler, for discovery, characterization, and engineering of natural product biosynthetic pathways (Shao, Luo, & Zhao, 2011). By taking advantage of the highly efficient yeast in vivo homologous recombination mechanism, this method synthesizes the entire expression vector containing the target biosynthetic pathway and the genetic elements needed for DNA maintenance and replication in individual hosts in a single-step manner. In this chapter, we describe the general guidelines for construct design. By using two distinct biosynthetic pathways, we demonstrate that DNA assembler can perform multiple tasks, including heterologous expression, introduction of single or multiple point mutations, scar-less gene deletion, generation of product derivatives, and creation of artificial gene clusters. As such, this method offers unprecedented flexibility and versatility in pathway manipulations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.