ArticleBiophysical journal2012
STICCS reveals matrix-dependent adhesion slipping and gripping in migrating cells.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Mapping Protein Diffusion from the Plasma Membrane to the Nucleus: Insights from Fluorescence Correlation Spectroscopy.Biomolecules · 2026Review
- Single-Cell Imaging of Mitochondrial Metabolism and Remodeling in C2C12 Murine Skeletal Muscle Cells upon Differentiation.International journal of molecular sciences · 2026Article
- Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.Biomolecules · 2026Article
- Quantifying superimposed protein flow dynamics in live cells using spatial filtering and spatiotemporal image correlation spectroscopy.Journal of microscopy · 2025Article
- Disruption of membrane cholesterol organization impairs the activity of PIEZO1 channel clusters.The Journal of general physiology · 2020Article
- Regulation and dynamics of force transmission at individual cell-matrix adhesion bonds.Science advances · 2020Article
- Cellular tension encodes local Src-dependent differential βMolecular biology of the cell · 2019Article
- Visualizing the Contribution of Keratin-14Stem cell reports · 2019Article
- PAM: A Framework for Integrated Analysis of Imaging, Single-Molecule, and Ensemble Fluorescence Data.Biophysical journal · 2018Article
- β-tubulin carboxy-terminal tails exhibit isotype-specific effects on microtubule dynamics in human gene-edited cells.Life science alliance · 2018Article
- Live-Cell Super-resolution Reveals F-Actin and Plasma Membrane Dynamics at the T Cell Synapse.Biophysical journal · 2017Article
- Article
- Intracellular kinetics of the androgen receptor shown by multimodal Image Correlation Spectroscopy (mICS).Scientific reports · 2016Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- An Intermittent Model for Intracellular Motions of Gold Nanostars by k-Space Scattering Image Correlation.Biophysical journal · 2015Article
- Characterization of Porous Materials by Fluorescence Correlation Spectroscopy Super-resolution Optical Fluctuation Imaging.ACS nano · 2015Article
- Far-red organic fluorophores contain a fluorescent impurity.Chemphyschem : a European journal of chemical physics and physical chemistry · 2014Article
- Review
- Dynamics of adhesion molecule domains on neutrophil membranes: surfing the dynamic cell topography.European biophysics journal : EBJ · 2013Article
- Fluctuation imaging spiced up with a piece of PIE.Biophysical journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Two-color spatio-temporal image cross-correlation spectroscopy (STICCS) is a new, to our knowledge, image analysis method that calculates space-time autocorrelation and cross-correlation functions from fluorescence intensity fluctuations. STICCS generates cellular flow and diffusion maps that reveal interactions and cotransport of two distinct molecular species labeled with different fluorophores. Here we use computer simulations to map the capabilities and limitations of STICCS for measurements in complex heterogeneous environments containing micro- and macrostructures. We then use STICCS to analyze the co-flux of adhesion components in migrating cells imaged using total internal reflection fluorescence microscopy. The data reveal a robust, time-dependent co-fluxing of certain integrins and paxillin in adhesions in protrusions when they pause, and in adhesions that are sliding and disassembling, demonstrating that the molecules in these adhesions move as a complex. In these regions, both α6β1- or αLβ2-integrins, expressed in CHO.B2 cells, co-flux with paxillin; an analogous cotransport was seen for α6β1-integrin and α-actinin in U2OS. This contrasts with the behavior of the α5β1-integrin and paxillin, which do not co-flux. Our results clearly show that integrins can move in complexes with adhesion proteins in protrusions that are retracting.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.