ArticleBiomedical optics express2012
Effect of molecular organization on the image histograms of polarization SHG microscopy.
Article in Biomedical optics express, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Characterization of Collagen Fiber Organization in Breast Cancer via Model-Free Multiscale pSHG Image Analysis.Annals of biomedical engineering · 2026Article
- Characterization of inner ear granular bodies by polarization-resolved second harmonic generation microscopy.Biomedical optics express · 2026Article
- Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy.Scientific reports · 2025Article
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Investigation into the structure of crystalline maltodextrin particles by second harmonic generation microscopy.Biomedical optics express · 2023Article
- Second harmonic generation microscopy of otoconia.Biomedical optics express · 2022Article
- Stokes polarimetry-based second harmonic generation microscopy for collagen and skeletal muscle fiber characterization.Lasers in medical science · 2021Article
- Quantitative structural imaging of keratoconic corneas using polarization-resolved SHG microscopy.Biomedical optics express · 2021Article
- Three-dimensional characterization of collagen remodeling in cell-seeded collagen scaffolds via polarization second harmonic generation.Biomedical optics express · 2021Article
- High-speed, multi-modal, label-free imaging of pathological slices with a Bessel beam.Biomedical optics express · 2020Article
- Identifying crossing collagen fibers in human corneal tissues using pSHG images.Biomedical optics express · 2019Article
- Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques.Methods and protocols · 2019Article
- Label-Free Fried Starchy Matrix: Investigation by Harmonic Generation Microscopy.Sensors (Basel, Switzerland) · 2019Article
- Harmonic Generation Microscopy 2.0: New Tricks Empowering Intravital Imaging for Neuroscience.Frontiers in molecular biosciences · 2019Review
- Whole-Section Tumor Micro-Architecture Analysis by a Two-Dimensional Phasor-Based Approach Applied to Polarization-Dependent Second Harmonic Imaging.Frontiers in oncology · 2019Article
- Polarized light microscopy for 3-dimensional mapping of collagen fiber architecture in ocular tissues.Journal of biophotonics · 2018Article
- Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration.Biomedical optics express · 2015Article
- Translational label-free nonlinear imaging biomarkers to classify the human corneal microstructure.Biomedical optics express · 2015Article
- Fast monitoring of in-vivo conformational changes in myosin using single scan polarization-SHG microscopy.Biomedical optics express · 2014Article
- Stokes vector based polarization resolved second harmonic microscopy of starch granules.Biomedical optics express · 2013Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Based on its polarization dependency, second harmonic generation (PSHG) microscopy has been proven capable to structurally characterize molecular architectures in different biological samples. By exploiting this polarization dependency of the SHG signal in every pixel of the image, average quantitative structural information can be retrieved in the form of PSHG image histograms. In the present study we experimentally show how the PSHG image histograms can be affected by the organization of the SHG active molecules. Our experimental scenario grounds on two inherent properties of starch granules. Firstly, we take advantage of the radial organization of amylopectin molecules (the SHG source in starch) to attribute shifts of the image histograms to the existence of tilted off the plane molecules. Secondly, we use the property of starch to organize upon hydration to demonstrate that the degree of structural order at the molecular level affects the width of the PSHG image histograms. The shorter the width is the more organized the molecules in the sample are, resulting in a reliable method to measure order. The implication of this finding is crucial to the interpretation of PSHG images used for example in tissue diagnostics.
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