ArticleJournal of bioenergetics and biomembranes2013
Characterization of functionally distinct mitochondrial subpopulations.
Article in Journal of bioenergetics and biomembranes, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Specialized high-capacity mitochondria fuel cell invasion.Current biology : CB · 2026Article
- Assessing Drug-Induced Mitochondrial Toxicity in Cardiomyocytes: Implications for Preclinical Cardiac Safety Evaluation.Pharmaceutics · 2022Review
- Anastasis: Return Journey from Cell Death.Cancers · 2021Review
- Reduction of host cell mitochondrial activity as Mycobacterium leprae's strategy to evade host innate immunity.Immunological reviews · 2021Review
- Respirometric Screening and Characterization of Mitochondrial Toxicants Within the ToxCast Phase I and II Chemical Libraries.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Assessment of Enrichment of Human Mesenchymal Stem Cells Based on Plasma and Mitochondrial Membrane Potentials.Bioelectricity · 2020Article
- An improved method for isolation of mitochondria from cell lines that enables reconstitution of calcium-dependent processes.Analytical biochemistry · 2019Article
- Mitochondrial Subtype Identification and Characterization.Current protocols in cytometry · 2018Article
- Affinity purification of cell-specific mitochondria from whole animals resolves patterns of genetic mosaicism.Nature cell biology · 2018Article
- Identification and Characterization of Mitochondrial Subtypes in Caenorhabditis elegans via Analysis of Individual Mitochondria by Flow Cytometry.Analytical chemistry · 2016Article
- Quantitative analysis of mitochondrial morphology and membrane potential in living cells using high-content imaging, machine learning, and morphological binning.Biochimica et biophysica acta · 2015Article
- Simultaneous measurement of individual mitochondrial membrane potential and electrophoretic mobility by capillary electrophoresis.Analytical chemistry · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mitochondrial stress results in changes in mitochondrial function, morphology and homeostasis (biogenesis, fission/fusion, mitophagy) and may lead to changes in mitochondrial subpopulations. While flow cytometric techniques have been developed to quantify features of individual mitochondria related to volume, Ca(2+) concentration, mtDNA content, respiratory capacity and oxidative damage, less information is available concerning the identification and characterization of mitochondrial subpopulations, particularly in epithelial cells. Mitochondria from rabbit kidneys were stained with molecular probes for cardiolipin content (nonyl acridine orange, NAO) and membrane potential (tetramethylrhodamine, TMRM) and analyzed using flow cytometry. We validated that side scatter was a better indicator of volume and that as side scatter (SSC) decreased mitochondrial volume increased. Furthermore, those mitochondria with the highest NAO content had greater side scattering and were most highly charged. Mitochondria with average NAO content were of average side scattering and maintained an intermediate charge. Those mitochondria with low NAO content had minimal side scattering and exhibited minimal charge. Upon titration with the uncoupler carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP), it was found that the high NAO content subpopulations were more resistant to uncoupling than lower NAO content populations. Ca(2+)-induced swelling of mitochondria was evaluated using probability binning (PB) analyses of SSC. Interestingly, only 30% of the mitochondria showed changes in response to Ca(2+), which was blocked by cyclosporine A. In addition, the small, high NAO content mitochondria swelled differentially in response to Ca(2+) over time. Our results demonstrate that flow cytometry can be used to identify mitochondrial subpopulations based on high, mid and low NAO content and/or volume/complexity. These subpopulations showed differences in membrane potential, volume, and responses to uncoupling and Ca(2+)-induced swelling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.