ArticleMolecular biology reports2013
Heterologous functional analysis of the Malus xiaojinensis MxIRT1 gene and the His-box motif by expression in yeast.
Article in Molecular biology reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- GsIRT3: a zinc-iron transporter gene from Glycine soja, enhancing alkali stress tolerance in Arabidopsis thaliana.Journal of plant research · 2026Article
- HRM and CRAC in MxIRT1 act as iron sensors to determine MxIRT1 vesicle-PM fusion and metal transport.Plant signaling & behavior · 2022Article
- Over-expression of the MxIRT1 gene increases iron and zinc content in rice seeds.Transgenic research · 2015Article
- Transcriptome profiling shows gene regulation patterns in a flavonoid pathway in response to exogenous phenylalanine in Boesenbergia rotunda cell culture.BMC genomics · 2014Article
- An uncleaved signal peptide directs the Malus xiaojinensis iron transporter protein Mx IRT1 into the ER for the PM secretory pathway.International journal of molecular sciences · 2014Article
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Authors and funding
7 authors.
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Abstract
Malus xiaojinensis is an important, iron-efficient rootstock germplasm. Iron uptake is an elaborately controlled process in plant roots, involving specialized transporters. MxIRT1, a Fe(II) transporter gene of M. xiaojinensis, is homologous to other iron transporters at the amino acid level. In the current study, the plasmid pYES2.0-MxIRT1, containing MxIRT1 cDNA, was constructed and transformed into yeast mutants. The results indicated that it could reverse the phenotype of yeast strain DEY1453, an iron uptake mutant. Complementation tests suggested that it might not be a specific transporter, as it was able to restore the phenotypes of other yeast mutant strains, including Mn, Cu and Zn uptake mutants. The functions of the critical histidine residues in the His-box of MxIRT1 were tested by transforming mutant yeast strain DEY1453 with different His residues altered by directed mutagenesis. The His-box of MxIRT1 was found to be necessary for iron transport, with different histidine residues (H(1-4)) playing different roles in the transport.
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