ArticleStem cells and development2013
An in vitro model of developmental synaptogenesis using cocultures of human neural progenitors and cochlear explants.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Engineering of functional auditory neurons from human induced pluripotent stem cells.Military Medical Research · 2026Article
- The Reconstruction of Peripheral Auditory Circuit: Recent Advances and Future Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Differentiation of Spiral Ganglion Neurons from Human Dental Pulp Stem Cells: A Further Step towards Autologous Auditory Nerve Recovery.International journal of molecular sciences · 2024Article
- Modelling inner ear development and disease using pluripotent stem cells - a pathway to new therapeutic strategies.Disease models & mechanisms · 2022Review
- Genetic Mechanism Study of Auditory Phoenix Spheres and Transcription Factors Prediction for Direct Reprogramming by Bioinformatics.International journal of molecular sciences · 2022Article
- In vitro and in vivo models: What have we learnt about inner ear regeneration and treatment for hearing loss?Molecular and cellular neurosciences · 2022Review
- Otic Organoids Containing Spiral Ganglion Neuron-like Cells Derived from Human-induced Pluripotent Stem Cells as a Model of Drug-induced Neuropathy.Stem cells translational medicine · 2022Article
- Progress in Modeling and Targeting Inner Ear Disorders with Pluripotent Stem Cells.Stem cell reports · 2020Review
- Altered expression of genes regulating inflammation and synaptogenesis during regrowth of afferent neurons to cochlear hair cells.PloS one · 2020Article
- Organotypic Culture of Neonatal Murine Inner Ear Explants.Frontiers in cellular neuroscience · 2019Article
- Organotypic Cocultures of Human Pluripotent Stem Cell Derived-Neurons with Mammalian Inner Ear Hair Cells and Cochlear Nucleus Slices.Stem cells international · 2019Article
- Reprogramming Glia Into Neurons in the Peripheral Auditory System as a Solution for Sensorineural Hearing Loss: Lessons From the Central Nervous System.Frontiers in molecular neuroscience · 2018Review
- Directed Differentiation of Human Embryonic Stem Cells Toward Placode-Derived Spiral Ganglion-Like Sensory Neurons.Stem cells translational medicine · 2017Article
- Single-Cell Transcriptome Analysis of Developing and Regenerating Spiral Ganglion Neurons.Current pharmacology reports · 2016Article
- Enriched retinal ganglion cells derived from human embryonic stem cells.Scientific reports · 2016Article
- Innervation of Cochlear Hair Cells by Human Induced Pluripotent Stem Cell-Derived Neurons In Vitro.Stem cells international · 2016Article
- Directing human induced pluripotent stem cells into a neurosensory lineage for auditory neuron replacement.BioResearch open access · 2014Article
- Prospects for replacement of auditory neurons by stem cells.Hearing research · 2013Review
- Stem-cell therapy for hearing loss: are we there yet?Brazilian journal of otorhinolaryngologyReview
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In mammals, the sensory hair cells and auditory neurons do not spontaneously regenerate and their loss results in permanent hearing impairment. Stem cell therapy is one emerging strategy that is being investigated to overcome the loss of sensory cells after hearing loss. To successfully replace auditory neurons, stem cell-derived neurons must be electrically active, capable of organized outgrowth of processes, and of making functional connections with appropriate tissues. We have developed an in vitro assay to test these parameters using cocultures of developing cochlear explants together with neural progenitors derived from human embryonic stem cells (hESCs). We found that these neural progenitors are electrically active and extend their neurites toward the sensory hair cells in cochlear explants. Importantly, this neurite extension was found to be significantly greater when neural progenitors were predifferentiated toward a neural crest-like lineage. When grown in coculture with hair cells only (denervated cochlear explants), stem cell-derived processes were capable of locating and growing along the hair cell rows in an en passant-like manner. Many presynaptic terminals (synapsin 1-positive) were observed between hair cells and stem cell-derived processes in vitro. These results suggest that differentiated hESC-derived neural progenitors may be useful for developing therapies directed at auditory nerve replacement, including complementing emerging hair cell regeneration therapies.
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