ArticleCellular microbiology2013
Diversion of phagosome trafficking by pathogenic Rhodococcus equi depends on mycolic acid chain length.
Article in Cellular microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Antimicrobial Resistance inAntibiotics (Basel, Switzerland) · 2026Review
- Trehalose dimycolate inhibits phagosome maturation and promotes intracellularProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A Photoactivatable Free Mycolic Acid Probe to Investigate Mycobacteria-Host Interactions.ACS infectious diseases · 2025Article
- Improved site-specific mutagenesis in Rhodococcus opacus using a novel conditional suicide plasmid.Applied microbiology and biotechnology · 2022Article
- The mycobacterial desaturase DesA2 is associated with mycolic acid biosynthesis.Scientific reports · 2022Article
- Identification of genes required for the fitness of Rhodococcus equi during the infection of mice via signature-tagged transposon mutagenesis.The Journal of veterinary medical science · 2021Article
- Effect of Macrolide and Rifampin Resistance on Fitness ofInfection and immunity · 2019Article
- The pathogenic actinobacterium Rhodococcus equi: what's in a name?Molecular microbiology · 2019Review
- Associating HEmerging microbes & infections · 2018Article
- Cyclopropane Modification of Trehalose Dimycolate Drives Granuloma Angiogenesis and Mycobacterial Growth through Vegf Signaling.Cell host & microbe · 2018Article
- The BCGΔBCG1419c Vaccine Candidate Reduces Lung Pathology, IL-6, TNF-α, and IL-10 During Chronic TB Infection.Frontiers in microbiology · 2018Article
- Article
- Trehalose dimycolate interferes with FcγR-mediated phagosome maturation through Mincle, SHP-1 and FcγRIIB signalling.PloS one · 2017Article
- The killing of macrophages by Corynebacterium ulcerans.Virulence · 2016Article
- Structure of the virulence-associated protein VapD from the intracellular pathogen Rhodococcus equi.Acta crystallographica. Section D, Biological crystallography · 2014Article
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
Abstract
Rhodococcus equi is a close relative of Mycobacterium spp. and a facultative intracellular pathogen which arrests phagosome maturation in macrophages before the late endocytic stage. We have screened a transposon mutant library of R. equi for mutants with decreased capability to prevent phagolysosome formation. This screen yielded a mutant in the gene for β-ketoacyl-(acyl carrier protein)-synthase A (KasA), a key enzyme of the long-chain mycolic acid synthesizing FAS-II system. The longest kasA mutant mycolic acid chains were 10 carbon units shorter than those of wild-type bacteria. Coating of non-pathogenic E. coli with purified wild-type trehalose dimycolate reduced phagolysosome formation substantially which was not the case with shorter kasA mutant-derived trehalose dimycolate. The mutant was moderately attenuated in macrophages and in a mouse infection model, but was fully cytotoxic.Whereas loss of KasA is lethal in mycobacteria, R. equi kasA mutant multiplication in broth was normal proving that long-chain mycolic acid compounds are not necessarily required for cellular integrity and viability of the bacteria that typically produce them. This study demonstrates a central role of mycolic acid chain length in diversion of trafficking by R. equi.
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