ArticleACS chemical neuroscience2012
Visualization of lipid raft membrane compartmentalization in living RN46A neuronal cells using single quantum dot tracking.
Article in ACS chemical neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Membrane staining and phospholipid tracking in Pseudomonas aeruginosa PAO1 using the phosphatidylcholine mimic propargyl-choline.Access microbiology · 2024Article
- Choosing the Probe for Single-Molecule Fluorescence Microscopy.International journal of molecular sciences · 2022Review
- Isolation of Lipid Rafts by the Detergent-Based and Non-detergent-Based Methods for Localization of GPCRs with Immunoblotting and Laser Scanning Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Bile Acids Gate Dopamine Transporter Mediated Currents.Frontiers in chemistry · 2021Article
- Single Quantum Dot Tracking Illuminates Neuroscience at the Nanoscale.Chemical physics letters · 2018Article
- A direct interaction of cholesterol with the dopamine transporter prevents its out-to-inward transition.PLoS computational biology · 2018Article
- Single-quantum-dot tracking reveals altered membrane dynamics of an attention-deficit/hyperactivity-disorder-derived dopamine transporter coding variant.ACS chemical neuroscience · 2015Article
- Quantum dots for quantitative imaging: from single molecules to tissue.Cell and tissue research · 2015Review
- Detection, counting, and imaging of single nanoparticles.Analytical chemistry · 2014Review
- Raft-like membranes from the trans-Golgi network and endosomal compartments.Nature protocols · 2013Article
- A Bright Light to Reveal Mobility: Single Quantum Dot Tracking Reveals Membrane Dynamics and Cellular Mechanisms.The journal of physical chemistry letters · 2013Article
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Lipid rafts are cholesterol-enriched subdomains in the plasma membrane that have been reported to act as a platform to facilitate neuronal signaling; however, they are suspected to have a very short lifetime, up to only a few seconds, which calls into question their roles in biological signaling. To better understand their diffusion dynamics and membrane compartmentalization, we labeled lipid raft constituent ganglioside GM1 with single quantum dots through the connection of cholera toxin B subunit, a protein that binds specifically to GM1. Diffusion measurements revealed that single quantum dot-labeled GM1 ganglioside complexes undergo slow, confined lateral diffusion with a diffusion coefficient of ∼7.87 × 10(-2) μm(2)/s and a confinement domain about 200 nm in size. Further analysis of their trajectories showed lateral confinement persisting on the order of tens of seconds, comparable to the time scales of the majority of cellular signaling and biological reactions. Hence, our results provide further evidence in support of the putative function of lipid rafts as signaling platforms.
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Registered trials
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