ArticlePloS one2012
Aging field collected Aedes aegypti to determine their capacity for dengue transmission in the southwestern United States.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- [Presence of Aedes aegypti in a high altitude area in Bolivia]Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina) · 2025Article
- Modelling the effects of adult emergence on the surveillance and age distribution of medically important mosquitoes.PLoS computational biology · 2025Article
- Impacts of ground-based ULV pyrethroid sprays on abundance and age structure of the Zika vector, Aedes aegypti (Diptera: Culicidae), in urban Arizona.Journal of medical entomology · 2025Article
- Estimation of population age structure, daily survival rates, and potential to support dengue virus transmission for Florida Keys Aedes aegypti via transcriptional profiling.PLoS neglected tropical diseases · 2024Article
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- Article
- Using body size as an indicator for age structure in field populations of Aedes aegypti (Diptera: Culicidae).Parasites & vectors · 2022Article
- A critical assessment of the detailed Aedes aegypti simulation model Skeeter Buster 2 using field experiments of indoor insecticidal control in Iquitos, Peru.PLoS neglected tropical diseases · 2022Article
- Article
- Use of transcriptional age grading technique to determine the chronological age of Sri Lankan Aedes aegypti and Aedes albopictus females.Parasites & vectors · 2021Article
- Prediction of mosquito species and population age structure using mid-infrared spectroscopy and supervised machine learning.Wellcome open research · 2019Article
- Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti.Scientific reports · 2017Article
- Efficacy of Some Wearable Devices Compared with Spray-On Insect Repellents for the Yellow Fever Mosquito, Aedes aegypti (L.) (Diptera: Culicidae).Journal of insect science (Online) · 2017Article
- Aedes aegypti (Diptera: Culicidae) Longevity and Differential Emergence of Dengue Fever in Two Cities in Sonora, Mexico.Journal of medical entomology · 2017Article
- Use of transgenicBulletin of the World Health Organization · 2016Article
- Naturally-acquired dengue virus infections do not reduce short-term survival of infected Aedes aegypti from Ho Chi Minh City, Vietnam.The American journal of tropical medicine and hygiene · 2015Article
- Parity and longevity of Aedes aegypti according to temperatures in controlled conditions and consequences on dengue transmission risks.PloS one · 2015Article
- The Efficacy of Some Commercially Available Insect Repellents for Aedes aegypti (Diptera: Culicidae) and Aedes albopictus (Diptera: Culicidae).Journal of insect science (Online) · 2015Article
- Effect of Larval Competition on Extrinsic Incubation Period and Vectorial Capacity of Aedes albopictus for Dengue Virus.PloS one · 2015Article
- Identification of morphological and chemical markers of dry- and wet-season conditions in female Anopheles gambiae mosquitoes.Parasites & vectors · 2014Article
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Authors and funding
7 authors.
Funding
Abstract
Aedes aegypti, the primary vector of dengue virus, is well established throughout urban areas of the Southwestern US, including Tucson, AZ. Local transmission of the dengue virus, however, has not been reported in this area. Although many factors influence the distribution of the dengue virus, we hypothesize that one contributing factor is that the lifespan of female Ae. aegypti mosquitoes in the Southwestern US is too short for the virus to complete development and be transmitted to a new host. To test this we utilized two age grading techniques. First, we determined parity by analyzing ovarian tracheation and found that only 40% of Ae. aegypti females collected in Tucson, AZ were parous. The second technique determined transcript levels of an age-associated gene, Sarcoplasmic calcium-binding protein 1 (SCP-1). SCP-1 expression decreased in a predictable manner as the age of mosquitoes increased regardless of rearing conditions and reproductive status. We developed statistical models based on parity and SCP-1 expression to determine the age of individual, field collected mosquitoes within three age brackets: nonvectors (0-5 days post-emergence), unlikely vectors (6-14 days post-emergence), and potential vectors (15+ days post-emergence). The statistical models allowed us to accurately group individual wild mosquitoes into the three age brackets with high confidence. SCP-1 expression levels of individual, field collected mosquitoes were analyzed in conjunction with parity status. Based on SCP-1 transcript levels and parity data, 9% of collected mosquitoes survived more than 15 days post emergence.
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