ArticlePloS one2012
Rapid and sensitive detection of Plesiomonas shigelloides by loop-mediated isothermal amplification of the hugA gene.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Performance evaluation of the Biosynex AMPLIQUICK Fecal Bacteriology PCR kit.BMC microbiology · 2025Article
- Rapid and Accurate Diagnosis of the Respiratory Disease Pertussis on a Point-of-Care Biochip.EClinicalMedicine · 2019Article
- Complete Genome Sequence of Multidrug-Resistant Plesiomonas shigelloides Strain MS-17-188.Genome announcements · 2018Article
- Rapid and sensitive detection of Plesiomonas shigelloides by cross‑priming amplification of the hugA gene.Molecular medicine reports · 2016Article
- Development and Diagnostic Evaluation of Loop-Mediated Isothermal Amplification Using a New Gene Target for Rapid Detection of Helicobacter pylori.Jundishapur journal of microbiology · 2016Article
- Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Aspergillus fumigatus.Journal of clinical microbiology · 2016Article
- Plesiomonas shigelloides Revisited.Clinical microbiology reviews · 2016Review
- Loop-mediated isothermal amplification for Rickettsia typhi (the causal agent of murine typhus): problems with diagnosis at the limit of detection.Journal of clinical microbiology · 2014Article
- Comparison of conventional PCR, multiplex PCR, and loop-mediated isothermal amplification assays for rapid detection of Arcobacter species.Journal of clinical microbiology · 2014Article
- Genome Sequence of Plesiomonas shigelloides Strain 302-73 (Serotype O1).Genome announcements · 2013Article
- Real-time fluorescence loop mediated isothermal amplification for the detection of Acinetobacter baumannii.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plesiomonas shigelloides is one of the causative agents of human gastroenteritis, with increasing number of reports describing such infections in recent years. In this study, the hugA gene was chosen as the target to design loop-mediated isothermal amplification (LAMP) assays for the rapid, specific, and sensitive detection of P. shigelloides. The performance of the assay with reference plasmids and spiked human stools as samples was evaluated and compared with those of quantitative PCR (qPCR). No false-positive results were observed for the 32 non-P. shigelloides strains used to evaluate assay specificity. The limit of detection for P. shigelloides was approximately 20 copies per reaction in reference plasmids and 5×10(3) CFU per gram in spiked human stool, which were more sensitive than the results of qPCR. When applied in human stool samples spiked with 2 low levels of P. shigelloides, the LAMP assays achieved accurate detection after 6-h enrichment. In conclusion, the LAMP assay developed in this study is a valuable method for rapid, cost-effective, and simple detection of P. shigelloides in basic clinical and field laboratories in the rural areas of China.
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