ArticleRadiology and oncology2012
miR-548c-5p inhibits proliferation and migration and promotes apoptosis in CD90(+) HepG2 cells.
Article in Radiology and oncology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Prediction of Age-Related MicroRNA Signature in Mesenchymal Stem Cells by using Computational Methods.Current stem cell research & therapy · 2025Article
- Exosomal long non-coding RNAs: novel molecules in gastrointestinal cancers' progression and diagnosis.Frontiers in oncology · 2022Review
- Advances in Liver Cancer Stem Cell Isolation and their Characterization.Stem cell reviews and reports · 2021Review
- Unravelling the role of long non-coding RNA -Non-coding RNA research · 2020Article
- Exosomal miR-548c-5p Regulates Colorectal Cancer Cell Growth and Invasion Through HIF1A/CDC42 Axis.OncoTargets and therapy · 2020Article
- MicroRNA expression profiles identify biomarkers for predicting the response to chemoradiotherapy in rectal cancer.Molecular medicine reports · 2018Article
- Association of miR-548c-5p, miR-7-5p, miR-210-3p, miR-128-3p with recurrence in systemically untreated breast cancer.Oncotarget · 2018Article
- Article
- MicroRNA regulation of liver cancer stem cells.American journal of cancer research · 2018Review
- Influence of selected anti-cancer drugs on the induction of DNA double-strand breaks and changes in gene expression in human hepatoma HepG2 cells.Environmental science and pollution research international · 2016Article
- CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA.Molecular cancer · 2015Article
- Fibroblasts from patients with major depressive disorder show distinct transcriptional response to metabolic stressors.Translational psychiatry · 2015Article
- Epigenetics in hepatocellular carcinoma: an update and future therapy perspectives.World journal of gastroenterology · 2014Review
- MiR-17-92 cluster: an apoptosis inducer or proliferation enhancer.Molecular and cellular biochemistry · 2013Article
- miRNA and TMPRSS2-ERG do not mind their own business in prostate cancer cells.Immunogenetics · 2013Review
- An miRNA Signature Predicts Grading of Pancreatic Neuroendocrine Neoplasms.Cancer genomics & proteomicsArticle
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSince the introduction of the theory of tumour stem cells (TSCs), the liver cancer stem cell (LCSC)-like cells have become one of the focuses in the research on liver cancer. MATERIALS AND METHODS.: In this study, CD90(+) cells were applied as the possible LCSC-like cells, and the miRNA and gene expression were analyzed in the CD90(+) HepG2 cells. The pilot study showed miR-548c-5p exerted potential effect on the CD90(+) HepG2 cells and was thereafter applied for the further study. CD90(+) HepG2 cells were assigned to miR-548c-5p mimic transfection group and control group. MTT assay was performed to detect the proliferation of CD90(+) HepG2 cells. The migration and invasion abilities were examined by wound healing assay and transwell migration assay, respectively. A detection of apoptosis was performed by fluorescence microscopy.
resultsOur results showed that caspase-3 and bcl-2 were down-regulated while caspase-8 was up-regulated in the CD90(+) HepG2 cells. Moreover, the miR-548c-5p transfection could down-regulate the expression of β-catenin, Tg737, bcl-2, bcl-XL, and caspase-3, inhibit the proliferation, migration and invasion and promote the apoptosis of the CD90(+) HepG2 cells.
conclusionsOur findings indicate the imbalance between apoptosis and anti-apoptosis in the LCSC-like cells, which influence the biological features of LCSC-like cells. miRNA plays a regulatory role in the LCSC-like cells among which miR-548c-5p might be a suppressor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.