ArticleProceedings of the National Academy of Sciences of the United States of America2012
Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 284 citations in OpenAlex.
- Sea Urchin-Inspired Immuno-Instructive Ionic Flow Drives MSCs-Macrophage Communication to Promote Bone Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Intracellular acid-base regulation mediates a trade-off between shell and somatic growth in a clam under ocean acidification.iScience · 2026Article
- Proton channels govern vesicular carbonate chemistry in mineralizing cells of a marine calcifier.Nature communications · 2026Article
- Intrinsic over extrinsic: Species identity shapes spatial and interannual Mg/Ca patterns in Arctic marine calcifiers.PloS one · 2026Article
- CMTM4 is an adhesion modulator that regulates skeletal patterning and primary mesenchyme cell migration in sea urchin embryos.Developmental biology · 2025Article
- Respiratory protein-driven selectivity during the Permian-Triassic mass extinction.Innovation (Cambridge (Mass.)) · 2024Article
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- Ten years of marine evolutionary biology-Challenges and achievements of a multidisciplinary research initiative.Evolutionary applications · 2023Article
- Extracellular carbonic anhydrase activity promotes a carbon concentration mechanism in metazoan calcifying cells.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Direct and latent effects of ocean acidification on the transition of a sea urchin from planktonic larva to benthic juvenile.Scientific reports · 2022Article
- Ovothiol ensures the correct developmental programme of the sea urchinOpen biology · 2022Article
- Ion Pathways in Biomineralization: Perspectives on Uptake, Transport, and Deposition of Calcium, Carbonate, and Phosphate.Journal of the American Chemical Society · 2021Article
- An otopetrin family proton channel promotes cellular acid efflux critical for biomineralization in a marine calcifier.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Intracellular pH regulation: characterization and functional investigation of HBMC molecular and cell biology · 2021Article
- Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking.Cells & development · 2021Article
- Gene expression patterns of red sea urchins (Mesocentrotus franciscanus) exposed to different combinations of temperature and pCOBMC genomics · 2021Article
- Patterns of Element Incorporation in Calcium Carbonate Biominerals Recapitulate Phylogeny for a Diverse Range of Marine Calcifiers.Frontiers in earth science · 2021Article
- Cellular pathways of calcium transport and concentration toward mineral formation in sea urchin larvae.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2020Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Calcifying echinoid larvae respond to changes in seawater carbonate chemistry with reduced growth and developmental delay. To date, no information exists on how ocean acidification acts on pH homeostasis in echinoderm larvae. Understanding acid-base regulatory capacities is important because intracellular formation and maintenance of the calcium carbonate skeleton is dependent on pH homeostasis. Using H(+)-selective microelectrodes and the pH-sensitive fluorescent dye BCECF, we conducted in vivo measurements of extracellular and intracellular pH (pH(e) and pH(i)) in echinoderm larvae. We exposed pluteus larvae to a range of seawater CO(2) conditions and demonstrated that the extracellular compartment surrounding the calcifying primary mesenchyme cells (PMCs) conforms to the surrounding seawater with respect to pH during exposure to elevated seawater pCO(2). Using FITC dextran conjugates, we demonstrate that sea urchin larvae have a leaky integument. PMCs and spicules are therefore directly exposed to strong changes in pH(e) whenever seawater pH changes. However, measurements of pH(i) demonstrated that PMCs are able to fully compensate an induced intracellular acidosis. This was highly dependent on Na(+) and HCO(3)(-), suggesting a bicarbonate buffer mechanism involving secondary active Na(+)-dependent membrane transport proteins. We suggest that, under ocean acidification, maintained pH(i) enables calcification to proceed despite decreased pH(e). However, this probably causes enhanced costs. Increased costs for calcification or cellular homeostasis can be one of the main factors leading to modifications in energy partitioning, which then impacts growth and, ultimately, results in increased mortality of echinoid larvae during the pelagic life stage.
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