ArticleHuman reproduction (Oxford, England)2013
Localization and identification of sumoylated proteins in human sperm: excessive sumoylation is a marker of defective spermatozoa.
Article in Human reproduction (Oxford, England), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 69 citations in OpenAlex.
- Biomarker-based human and animal sperm phenotyping: the good, the bad and the ugly†.Biology of reproduction · 2024Review
- Phosphoproteome analysis of the crosstalk between sumoylation and phosphorylation in mouse spermatocytes.Biochemical and biophysical research communications · 2023Article
- SUMO1 and Defective Spermatozoa Correlate with Endogenous Hydrogen Peroxide and Live Birth Outcome in Intrauterine Insemination Cycles for Unexplained Infertility.International journal of molecular sciences · 2023Article
- Implementing a preimplantation proteomic approach to advance assisted reproduction technologies in the framework of predictive, preventive, and personalized medicine.The EPMA journal · 2022Review
- Female-induced selective modification of sperm protein SUMOylation-potential mechanistic insights into the non-random fertilization in humans.Journal of evolutionary biology · 2022Article
- Sumoylation and its regulation in testicular Sertoli cells.Biochemical and biophysical research communications · 2021Article
- TMT-based proteomic and bioinformatic analyses of human granulosa cells from obese and normal-weight female subjects.Reproductive biology and endocrinology : RB&E · 2021Article
- Male infertility is not liked with HSF1, HSF2 and UBE2I gene polymorphisms among Indian subjects.Bioinformation · 2021Article
- Hypoxia-induced Changes in SUMO Conjugation Affect Transcriptional Regulation Under Low Oxygen.Molecular & cellular proteomics : MCP · 2019Article
- SUMO targeting of a stress-tolerant Ulp1 SUMO protease.PloS one · 2018Article
- Stromal Senp1 promotes mouse early folliculogenesis by regulating BMP4 expression.Cell & bioscience · 2017Article
- Expression, Localization of SUMO-1, and Analyses of Potential SUMOylated Proteins inFrontiers in physiology · 2017Article
- Identification of cell-specific targets of sumoylation during mouse spermatogenesis.Reproduction (Cambridge, England) · 2016Article
- Regulation of germ cell function by SUMOylation.Cell and tissue research · 2016Review
- Proteomic approaches for profiling negative fertility markers in inferior boar spermatozoa.Scientific reports · 2015Article
- Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins.Analytical biochemistry · 2015Article
- CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.Toxicology letters · 2015Article
- High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer.American journal of cancer research · 2015Article
- Target antigens for Hs-14 monoclonal antibody and their various expression in normozoospermic and asthenozoospermic men.Basic and clinical andrology · 2015Article
- High-level SAE2 promotes malignant phenotype and predicts outcome in gastric cancer.American journal of cancer research · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundSumoylation is a type of post-translational modification that is implicated in the regulation of numerous cellular events. However, its role in the function of human sperm has not yet been characterized. METHODS AND
resultsIn this study, both immunofluorescence and electron microscopy revealed that small ubiquitin-like modifiers (SUMO) SUMO1 and SUMO2/3 were highly enriched in the neck area of human sperm that is associated with the redundant nuclear envelope and were also detectable in the flagella and some head regions. Similar localization patterns of SUMO were also observed in mouse and fly sperm. Nonmotile, two-tailed, curled tailed, misshapen, microcephalic (small head) and aciphalic (no head) sperm exhibited abnormally high levels of sumoylation in their neck and tail regions relative to normal sperm. Numerous sumoylated proteins, ranging from 20 to 260 kDa, were detected via western blotting and identified by mass spectrometry, and 55 SUMO targets that were present specifically in human sperm, and not in the control fraction, corresponded to flagella proteins, proteins involved in the maturation and differentiation of sperm, heat shock proteins and important glycolytic and mitochondrial enzymes. The targets that were identified included proteins with specific functions in germ cells and sperm, such as heat shock-related 70-kDa protein 2, outer dense fiber protein 3, A-kinase anchor proteins 3 and 4, L-lactate dehydrogenase C, sperm protein associated with the nucleus on the X chromosome B/F, valosin-containing protein, seminogelins, histone H4 and ubiquitin. Coimmunoprecipitation experiments confirmed the sumoylation of semenogelin and indicated that some sperm proteins are modified by sumoylation and ubiquitination simultaneously.
conclusionsNumerous proteins are modified by sumoylation in human sperm; excessive sumoylation is a marker of defective spermatozoa.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.