ArticleJournal of clinical microbiology2013
Neutralizing DNA aptamers against swine influenza H3N2 viruses.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Nucleic Acid Aptamer-Based Biosensors: A Review.Biomedicines · 2023Review
- Development of a one-shot dual aptamer-based fluorescence nanosensor for rapid, sensitive, and label-free detection of periostin.Scientific reports · 2023Article
- Identification of G-quadruplex anti-Interleukin-2 aptamer with high specificity through SELEX stringency.Heliyon · 2022Article
- ProNAB: database for binding affinities of protein-nucleic acid complexes and their mutants.Nucleic acids research · 2022Article
- Aptamers in Virology-A Consolidated Review of the Most Recent Advancements in Diagnosis and Therapy.Pharmaceutics · 2021Review
- Aptamer-based diagnostic and therapeutic approaches in animals: Current potential and challenges.Saudi journal of biological sciences · 2021Review
- Potential applications of aptamers in veterinary science.Veterinary research · 2021Review
- Emerging Technologies for the Treatment of COVID-19.Advances in experimental medicine and biology · 2021Article
- Oligonucleotide aptamers: promising and powerful diagnostic and therapeutic tools for infectious diseases.The Journal of infection · 2018Review
- Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEXIranian biomedical journal · 2017Article
- Complexes of Oligoribonucleotides with D-Mannitol Inhibit Hemagglutinin-Glycan Interaction and Suppress Influenza A Virus H1N1 (A/FM/1/47) Infectivity In Vitro.Pharmaceuticals (Basel, Switzerland) · 2017Article
- Application of aptamers in diagnostics, drug-delivery and imaging.Journal of biosciences · 2016Article
- Aptasensors for viral diagnostics.Trends in analytical chemistry : TRAC · 2015Review
- Cross-protection of influenza A virus infection by a DNA aptamer targeting the PA endonuclease domain.Antimicrobial agents and chemotherapy · 2015Article
- A review on emerging diagnostic assay for viral detection: the case of avian influenza virus.Molecular biology reports · 2015Review
- Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications.BioMed research international · 2015Review
- Two DNA aptamers against avian influenza H9N2 virus prevent viral infection in cells.PloS one · 2015Article
- Clinical differences between H3N2 and H1N1 influenza 2012 and lower respiratory tract infection found using a statistical classification approach.Therapeutics and clinical risk management · 2014Article
- A combined enrichment and aptamer pulldown assay for Francisella tularensis detection in food and environmental matrices.PloS one · 2014Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Triple reassortant influenza A viruses (IAVs) of swine, particularly the North American H3N2 subtype, circulate in swine herds and may reassort and result in the emergence of novel zoonotic strains. Current diagnostic tools rely on isolation of the viruses, followed by serotyping by hemagglutination or genome sequencing, both of which can be expensive and time-consuming. Thus, novel subtype-specific ligands and methods are needed for rapid testing and subtyping of IAVs in the field. To address this need, we selected DNA aptamers against the recombinant HA protein from swine IAV H3 cluster IV using systematic evolution of ligands by exponential enrichment (SELEX). Four candidate aptamers (HA68, HA7, HA2a, and HA2b) were identified and characterized. The dissociation constants (K(d)) of aptamers HA68, HA7, HA2a, and HA2b against recombinant H3 protein were 7.1, 22.3, 16.0, and 3.7 nM, respectively. The binding site of HA68 to H3 was identified to be between nucleotide residues 8 and 40. All aptamers inhibited H3 hemagglutination. HA68 was highly specific to all four lineages within the North American H3N2 subtype. Further, the other three aptamers specifically identified live viruses belonging to the phylogenetic clusters I, II/III, and IV especially the virus that closely related to the recent H3N2 variant (H3N2v). Aptamer HA68 was also able to bind and detect H3N2v isolated from recent human cases. In conclusion, we provide subtype-specific aptamers against H3N2 IAVs of swine that can now be used in rapid detection and typing protocols for field applications.
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