ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Compartmentalization and Ca2+ buffering are essential for prevention of light-induced retinal degeneration.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
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- Trichoplusia ni Transcriptomic Responses to the Phytosaponin Aglycone Hederagenin: Sex-Related Differences.Journal of chemical ecology · 2024Article
- Single-cell profiling reveals Müller glia coordinate retinal intercellular communication during light/dark adaptation via thyroid hormone signaling.Protein & cell · 2023Article
- Aging and Light Stress Result in Overlapping and Unique Gene Expression Changes in Photoreceptors.Genes · 2022Article
- Discovery and Visualization of Age-Dependent Patterns in the Diurnal Transcriptome of Drosophila.Journal of circadian rhythms · 2022Article
- Knockdown of Dehydrodolichyl Diphosphate Synthase in theFrontiers in molecular neuroscience · 2021Article
- Blue light induces a neuroprotective gene expression program in Drosophila photoreceptors.BMC neuroscience · 2018Article
- Transcriptome profiling of aging Drosophila photoreceptors reveals gene expression trends that correlate with visual senescence.BMC genomics · 2017Article
- Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors.Journal of visualized experiments : JoVE · 2017Article
- Ectopic Expression of Mouse Melanopsin inThe Journal of biological chemistry · 2017Article
- Electroretinogram (ERG) Recordings fromBio-protocol · 2015Article
- Functional cooperation between the IP3 receptor and phospholipase C secures the high sensitivity to light of Drosophila photoreceptors in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- A new genetic model for calcium induced autophagy and ER-stress in Drosophila photoreceptor cells.Channels (Austin, Tex.) · 2015Article
- Fractional Ca(2+) currents through TRP and TRPL channels in Drosophila photoreceptors.Biophysical journal · 2013Article
- Separate Ca2+ sources are buffered by distinct Ca2+ handling systems in aplysia neuroendocrine cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Fly photoreceptors are polarized cells, each of which has an extended interface between its cell body and the light-signaling compartment, the rhabdomere. Upon intense illumination, rhabdomeric calcium concentration reaches millimolar levels that would be toxic if Ca(2+) diffusion between the rhabdomere and cell body was not robustly attenuated. Yet, it is not clear how such effective attenuation is obtained. Here we show that Ca(2+) homeostasis in the photoreceptor cell relies on the protein calphotin. This unique protein functions as an immobile Ca(2+) buffer localized along the base of the rhabdomere, separating the signaling compartment from the cell body. Generation and analyses of transgenic Drosophila strains, in which calphotin-expression levels were reduced in a graded manner, showed that moderately reduced calphotin expression impaired Ca(2+) homeostasis while calphotin elimination resulted in severe light-dependent photoreceptor degeneration. Electron microscopy, electrophysiology, and optical methods revealed that the degeneration was rescued by prevention of Ca(2+) overload via overexpression of CalX, the Na(+)-Ca(2+) exchanger. In addition, Ca(2+)-imaging experiments showed that reduced calphotin levels resulted in abnormally fast kinetics of Ca(2+) elevation in photoreceptor cells. Together, the data suggest that calphotin functions as a Ca(2+) buffer; a possibility that we directly demonstrate by expressing calphotin in a heterologous expression system. We propose that calphotin-mediated compartmentalization and Ca(2+) buffering constitute an effective strategy to protect cells from Ca(2+) overload and light-induced degeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.