ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Fast scan cyclic voltammetry as a novel method for detection of real-time gonadotropin-releasing hormone release in mouse brain slices.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed, 61 citations in OpenAlex.
- Functional interactions between anteroventral periventricular kisspeptin neurons and gonadotropin-releasing hormone neurons in female mice.Journal of neuroendocrinology · 2026Article
- Pyrolyzed Parylene Electrodes for Detection of Tryptophan, Tyrosine, and Gonadotropin-Releasing Hormone.ACS measurement science au · 2026Article
- Altered Electrophysiology and Transcriptome of GnRH Neurons in Middle-Aged Female Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Estradiol action in the female hypothalamo-pituitary-gonadal axis.Journal of neuroendocrinology · 2024Review
- Editors' Choice-Review-The Future of Carbon-Based Neurochemical Sensing: A Critical Perspective.ECS sensors plus · 2023Review
- Gonadal Feedback Alters the Relationship between Action Potentials and Hormone Release in Gonadotropin-Releasing Hormone Neurons in Male Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- Measuring tryptophan dynamics using fast scan cyclic voltammetry at carbon fiber microelectrodes with improved sensitivity and selectivity.RSC advances · 2023Article
- Estrogens and the circadian system.Seminars in cell & developmental biology · 2022Review
- The electrophysiologic properties of gonadotropin-releasing hormone neurons.Journal of neuroendocrinology · 2022Review
- Mutual Interactions Between GnRH and Kisspeptin in GnRH- and Kiss-1-Expressing Immortalized Hypothalamic Cell Models.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Sexually Dimorphic Neurosteroid Synthesis Regulates Neuronal Activity in the Murine Brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Role of core circadian clock genes in hormone release and target tissue sensitivity in the reproductive axis.Molecular and cellular endocrinology · 2020Review
- Differential Roles of Hypothalamic AVPV and Arcuate Kisspeptin Neurons in Estradiol Feedback Regulation of Female Reproduction.Neuroendocrinology · 2020Review
- Central aspects of systemic oestradiol negative- and positive-feedback on the reproductive neuroendocrine system.Journal of neuroendocrinology · 2020Review
- Dopamine Release Dynamics in the Tuberoinfundibular Dopamine System.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- Changes in Both Neuron Intrinsic Properties and Neurotransmission Are Needed to Drive the Increase in GnRH Neuron Firing Rate during Estradiol-Positive Feedback.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Modulation of Gonadotropin-Releasing Hormone Neuron Activity and Secretion in Mice by Non-peptide Neurotransmitters, Gasotransmitters, and Gliotransmitters.Frontiers in endocrinology · 2019Review
- Identification of Genes Enriched in GnRH Neurons by Translating Ribosome Affinity Purification and RNAseq in Mice.Endocrinology · 2018Article
- Glutamatergic Transmission to Hypothalamic Kisspeptin Neurons Is Differentially Regulated by Estradiol through Estrogen Receptor α in Adult Female Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Pulsatile gonadotropin-releasing hormone (GnRH) release is critical for the central regulation of fertility. There is no method allowing real-time GnRH detection in brain slices. We developed fast-scan cyclic voltammetry (FSCV) using carbon-fiber microelectrodes (CFME) to detect GnRH release and validated it using a biologically relevant system. FSCV parameters (holding potential, switching potential, and scan rate) were determined for stable GnRH detection in vitro, then optimized for GnRH detection in mouse brain slices. Placement of CFMEs in the median eminence (ME) near GnRH terminals allowed detection of both KCl-evoked and spontaneous GnRH release. GnRH release was also detected from GnRH fibers passing near GnRH soma and near fiber-fiber appositions in the preoptic area. No GnRH signal was detected from CFMEs in the ME of hpg mice, which lack GnRH, or in regions not containing GnRH neurons in wild-type mice; application of exogenous GnRH produced a signal similar to that observed for spontaneous/evoked endogenous GnRH release. Using an established mouse model that produces diurnal variations in GnRH neuron activity, we demonstrated corresponding changes in spontaneous GnRH release in the median eminence. These results validate FSCV to detect GnRH in brain slices and provide new information on the sites and amounts of GnRH release, providing insight into its neuromodulatory functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.