ArticleThe Journal of cell biology1990
Heterogeneity of mRNA and protein products arising from the protein 4.1 gene in erythroid and nonerythroid tissues.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 122 citations in OpenAlex.
- Alternative polyadenylation in a family of paralogous EPB41 genes generates protein 4.1 diversity.RNA biology · 2017Article
- Interaction of 4.1G and cGMP-gated channels in rod photoreceptor outer segments.Journal of cell science · 2013Article
- NMR characterisation of the minimal interacting regions of centrosomal proteins 4.1R and NuMA1: effect of phosphorylation.BMC biochemistry · 2010Article
- Article
- Inhibition of protein 4.1 R and NuMA interaction by mutagenization of their binding-sites abrogates nuclear localization of 4.1 R.Clinical and translational science · 2009Article
- Alternatively spliced exon 5 of the FERM domain of protein 4.1R encodes a novel binding site for erythrocyte p55 and is critical for membrane targeting in epithelial cells.Biochimica et biophysica acta · 2009Article
- An internal ribosome entry site element directs the synthesis of the 80 kDa isoforms of protein 4.1R.BMC biology · 2008Article
- Regulated Fox-2 isoform expression mediates protein 4.1R splicing during erythroid differentiation.Blood · 2008Article
- Protein 4.1R self-association: identification of the binding domain.The Biochemical journal · 2006Article
- Cardiac muscle cell cytoskeletal protein 4.1: analysis of transcripts and subcellular location--relevance to membrane integrity, microstructure, and possible role in heart failure.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Mitotic regulation of protein 4.1R involves phosphorylation by cdc2 kinase.Molecular biology of the cell · 2005Article
- Identification of a novel microtubule-destabilizing motif in CPAP that binds to tubulin heterodimers and inhibits microtubule assembly.Molecular biology of the cell · 2004Article
- A nonerythroid isoform of protein 4.1R interacts with components of the contractile apparatus in skeletal myofibers.Molecular biology of the cell · 2000Article
- Protein 4.1 R-135 interacts with a novel centrosomal protein (CPAP) which is associated with the gamma-tubulin complex.Molecular and cellular biology · 2000Article
- The N-terminal 209-aa domain of high molecular-weight 4.1R isoforms abrogates 4.1R targeting to the nucleus.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- A novel neuron-enriched homolog of the erythrocyte membrane cytoskeletal protein 4.1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Deciphering the nuclear import pathway for the cytoskeletal red cell protein 4.1R.Molecular biology of the cell · 1999Article
- A nonerythroid isoform of protein 4.1R interacts with the nuclear mitotic apparatus (NuMA) protein.The Journal of cell biology · 1999Article
- Protein 4.1R-deficient mice are viable but have erythroid membrane skeleton abnormalities.The Journal of clinical investigation · 1999Article
- Structural protein 4.1 is located in mammalian centrosomes.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Immunologically cross-reactive isoforms of the cytoskeletal element protein 4.1 have been identified in many tissues in which they exhibit heterogeneity of molecular weight, abundance, and intracellular localization. To examine the basis for isoform production in erythroid and nonerythroid tissues, we have compared the structure and expression of cDNAs isolated from human erythroid and nonerythroid sources. We have encountered cDNAs representing many distinct mRNA sequences. These exhibit complete nucleotide sequence homology along most of their lengths. Differences were confined to five sequence blocks designated Motifs I-V, which were present or absent in each mRNA moiety. Motif I was expressed only in erythroid cells; it encodes 21 amino acids in a well-characterized spectrin/actin binding domain. Motif II, located near the COOH terminus of the 80-kD "erythroid" protein 4.1 molecule is present in the vast majority of transcripts from both erythroid and nonerythroid cells. Motifs IV and V alter the 5' untranslated region: simultaneous insertion of Motif IV and deletion of Motif V in the untranslated region inserts a new initiator methionine and establishes a contiguous open reading frame encoding a novel 135-kD protein 4.1 molecule. By immunochemical analysis we have identified the longer isoform in cells. Our results are most consistent with tissue-specific alternative mRNA splicing of transcripts of the protein 4.1 gene to yield numerous isoforms. These isoforms exhibit tissue specificity and alter strategic portions of the molecule. Moreover, we describe a novel high molecular weight form of protein 4.1 that arises by splicing events which allow translation at an upstream site.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.