ArticleThe Journal of cell biology1990
An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 126 citations in OpenAlex.
- Shiga toxin is transported into the nucleoli of intestinal epithelial cells via a carrier-dependent process.Toxins · 2010Article
- The N-terminal double-stranded RNA binding domains of Arabidopsis HYPONASTIC LEAVES1 are sufficient for pre-microRNA processing.The Plant cell · 2007Article
- A novel fluorescence-based genetic strategy identifies mutants of Saccharomyces cerevisiae defective for nuclear pore complex assembly.Molecular biology of the cell · 1998Article
- Negative regulation of DNA replication by the retinoblastoma protein is mediated by its association with MCM7.Molecular and cellular biology · 1998Article
- The integral membrane protein snl1p is genetically linked to yeast nuclear pore complex function.Molecular biology of the cell · 1998Article
- Nucleocytoplasmic recycling of the nuclear localization signal receptor alpha subunit in vivo is dependent on a nuclear export signal, energy, and RCC1.The Journal of cell biology · 1997Article
- Functional domains in nuclear import factor p97 for binding the nuclear localization sequence receptor and the nuclear pore.Molecular biology of the cell · 1997Article
- A nuclear export signal in Kap95p is required for both recycling the import factor and interaction with the nucleoporin GLFG repeat regions of Nup116p and Nup100p.The Journal of cell biology · 1997Article
- In vivo dynamics of nuclear pore complexes in yeast.The Journal of cell biology · 1997Article
- Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.The Journal of cell biology · 1996Article
- Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.The Journal of cell biology · 1995Article
- Mutants in a yeast Ran binding protein are defective in nuclear transport.The EMBO journal · 1995Article
- Early events in DNA replication require cyclin E and are blocked by p21CIP1.The Journal of cell biology · 1995Article
- In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import.The EMBO journal · 1995Article
- Sequence and characterization of cytoplasmic nuclear protein import factor p97.The Journal of cell biology · 1995Article
- Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62.The Journal of cell biology · 1995Article
- Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.The Journal of cell biology · 1995Article
- The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus.The Journal of cell biology · 1995Article
- Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex.The Journal of cell biology · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We described previously an assay for authentic nuclear protein import in vitro. In this assay, exogenous nuclei are placed in an extract of Xenopus eggs; a rhodamine-labeled protein possessing a nuclear localization signal is added, and fluorescence microscopy is used to measure nuclear uptake. The requirement in this system for a cytosolic extract suggests that nuclear import is dependent on at least one cytosolic factor. We now confirm this hypothesis. Treatment of the cytosol with N-ethylmaleimide (NEM) abolishes nuclear protein import; readdition of a cytosolic fraction to the NEM-inactivated extract rescues transport. Thus, at least one NEM-sensitive factor required for transport is supplied by the cytosol. This activity, called nuclear import factor-1, or NIF-1, is ammonium-sulfate-precipitable, protease-sensitive, and heat-labile; it is therefore at least partly proteinaceous. NIF-1 stimulates, in a concentration-dependent manner, the rate at which individual nuclei accumulate protein. The effect of NIF-1 is enhanced by a second cytosolic NEM-sensitive factor, NIF-2. Earlier we identified two steps in the nuclear import reaction: (a) ATP-independent binding of a signal-sequence-bearing protein to the nuclear pore; and (b) ATP-dependent translocation of that protein through the pore. We now show that NEM inhibits signal-mediated binding, and that readdition of NIF-1 restores binding. Thus, NIF-1 is required for at least the binding step and does not require ATP for its activity. NIF-1 may act as a cytoplasmic signal receptor that escorts signal-bearing proteins to the pore, or may instead promote signal-mediated binding to the pore in another manner, as discussed.
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