ArticleBMC microbiology2012
Enhancement of carotenoid production by disrupting the C22-sterol desaturase gene (CYP61) in Xanthophyllomyces dendrorhous.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 63 citations in OpenAlex.
- Functional Characterization of a Signal Peptide Peptidase inInternational journal of molecular sciences · 2026Article
- Astaxanthin biosynthesis stimulation in wild type Xanthophyllomyces dendrorhous allows transcriptional reprogramming of genetic responses involved in oxidative stress and mitochondrial defense.World journal of microbiology & biotechnology · 2026Article
- Comparative Transcriptomic Analyses Reveal Potential Stp1 Regulatory Roles Independent of Sre1 inInternational journal of molecular sciences · 2025Article
- Omics-driven onboarding of the carotenoid producing red yeast Xanthophyllomyces dendrorhous CBS 6938.Applied microbiology and biotechnology · 2024Article
- Increasing carotenoid production in Xanthophyllomyces dendrorhous/Phaffia rhodozyma: SREBP pathway activation and promoter engineering.Biological research · 2024Article
- Identification of Potential New Genes Related to the SREBP Pathway inBiomolecules · 2024Article
- Codon usage bias in yeasts and its correlation with gene expression, growth temperature, and protein structure.Frontiers in microbiology · 2024Article
- Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction.Journal of fungi (Basel, Switzerland) · 2023Review
- Unraveling the Molecular Basis of Mycosporine Biosynthesis in Fungi.International journal of molecular sciences · 2023Article
- Rapid Gene Target Tracking for Enhancing β-Carotene Production Using Flow Cytometry-Based High-Throughput Screening in Yarrowia lipolytica.Applied and environmental microbiology · 2022Article
- Role of ROX1, SKN7, and YAP6 Stress Transcription Factors in the Production of Secondary Metabolites inInternational journal of molecular sciences · 2022Article
- Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies.Synthetic and systems biotechnology · 2022Review
- Damage response protein 1 (Dap1) functions in the synthesis of carotenoids and sterols in Xanthophyllomyces dendrorhous.Journal of lipid research · 2022Article
- The SREBP (Sterol Regulatory Element-Binding Protein) pathway: a regulatory bridge between carotenogenesis and sterol biosynthesis in the carotenogenic yeast Xanthophyllomyces dendrorhous.Biological research · 2021Review
- Metabolic engineering for high yield synthesis of astaxanthin in Xanthophyllomyces dendrorhous.Microbial cell factories · 2021Review
- Effectively Improve the Astaxanthin Production by Combined Additives Regulating Different Metabolic Nodes inFrontiers in bioengineering and biotechnology · 2021Article
- Sterol regulatory element-binding protein Sre1 regulates carotenogenesis in the red yeastJournal of lipid research · 2020Article
- The Untapped Australasian Diversity of Astaxanthin-Producing Yeasts with Biotechnological Potential-Microorganisms · 2020Article
- Fungal Pigments: Potential Coloring Compounds for Wide Ranging Applications in Textile Dyeing.Journal of fungi (Basel, Switzerland) · 2020Review
- Metallopeptidase Stp1 activates the transcription factor Sre1 in the carotenogenic yeastJournal of lipid research · 2020Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundXanthophyllomyces dendrorhous is a basidiomycetous yeast that synthesizes astaxanthin, which is a carotenoid with a great biotechnological impact. The ergosterol and carotenoid synthesis pathways are derived from the mevalonate pathway, and in both pathways, cytochrome P450 enzymes are involved.
resultsIn this study, we isolated and described the X. dendrorhous CYP61 gene, which encodes a cytochrome P450 involved in ergosterol biosynthesis. This gene is composed of nine exons and encodes a 526 amino acid polypeptide that shares significant percentages of identity and similitude with the C22-sterol desaturase, CYP61, from other fungi. Mutants derived from different parental strains were obtained by disrupting the CYP61 gene with an antibiotic selection marker. These mutants were not able to produce ergosterol and accumulated ergosta-5,8,22-trien-3-ol and ergosta-5,8-dien-3-ol. Interestingly, all of the mutants had a more intense red color phenotype than their respective parental strains. The carotenoid composition was qualitatively and quantitatively analyzed by RP-HPLC, revealing that the carotenoid content was higher in the mutant strains without major changes in their composition. The expression of the HMGR gene, which encodes an enzyme involved in the mevalonate pathway (3-hydroxy-3-methylglutaryl-CoA reductase), was analyzed by RT-qPCR showing that its transcript levels are higher in the CYP61 mutants.
conclusionsThese results suggest that in X. dendrorhous, ergosterol regulates HMGR gene expression by a negative feedback mechanism and in this way; it contributes in the regulation of the carotenoid biosynthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.