ArticleCellular and molecular bioengineering2012
Separating Fluid Shear Stress from Acceleration during Vibrations in Vitro: Identification of Mechanical Signals Modulating the Cellular Response.
Article in Cellular and molecular bioengineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The trial behind it
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Who cites it
30 citing papers in PubMed.
- Low intensity vibration as a novel strategy to normalize age-related deficits in T cell proliferation, activation, and function.Scientific reports · 2026Article
- Increased deformations are dispensable for encapsulated cell mechanoresponse in engineered bone analogs mimicking aging bone marrow.Mechanobiology in medicine · 2025Article
- Low intensity mechanical signals promote proliferation in a cell-specific manner: Tailoring a non-drug strategy to enhance biomanufacturing yields.Mechanobiology in medicine · 2024Article
- Vibro-Acoustic Platelet Activation: An Additive Mechanism of Prothrombosis with Applicability to Snoring and Obstructive Sleep Apnea.Bioengineering (Basel, Switzerland) · 2023Article
- Increased deformations are dispensable for cell mechanoresponse in engineered bone analogs mimicking aging bone marrow.bioRxiv : the preprint server for biology · 2023Article
- Vertical Vibration of Mouse Osteoblasts Promotes Cellular Differentiation and Cell Cycle Progression and Induces Aging In Vitro.Biomedicines · 2023Article
- Transport-Associated Vibrational Stress Triggers Drug-Reversible Apoptosis and Cardiac Allograft Failure in Mice.IEEE journal of translational engineering in health and medicine · 2023Article
- Frequency-specific sensitivity of 3T3-L1 preadipocytes to low-intensity vibratory stimulus during adipogenesis.In vitro cellular & developmental biology. Animal · 2022Article
- Low Intensity Pulsed Ultrasound for Bone Tissue Engineering.Micromachines · 2021Review
- Review
- Low-intensity vibration restores nuclear YAP levels and acute YAP nuclear shuttling in mesenchymal stem cells subjected to simulated microgravity.NPJ microgravity · 2020Article
- Mechanical suppression of breast cancer cell invasion and paracrine signaling to osteoclasts requires nucleo-cytoskeletal connectivity.Bone research · 2020Article
- Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex.NPJ microgravity · 2019Article
- Bone changes after short-term whole body vibration are confined to cancellous bone.Journal of musculoskeletal & neuronal interactions · 2018Article
- Mechanically Loading Cell/Hydrogel Constructs with Low-Intensity Pulsed Ultrasound for Bone Repair.Tissue engineering. Part A · 2018Article
- [Influences of vibration on rapid osteogenic response of osteoblasts].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2017Article
- Vibrational stimulation induces osteoblast differentiation and the upregulation of osteogenic gene expression in vitro.Cytotechnology · 2016Article
- Cytoskeletal Configuration Modulates Mechanically Induced Changes in Mesenchymal Stem Cell Osteogenesis, Morphology, and Stiffness.Scientific reports · 2016Article
- Understanding Mechanobiology: Physical Therapists as a Force in Mechanotherapy and Musculoskeletal Regenerative Rehabilitation.Physical therapy · 2016Article
- Vibration Therapy to Prevent Bone Loss and Falls: Mechanisms and Efficacy.Current osteoporosis reports · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The identification of the physical mechanism(s) by which cells can sense vibrations requires the determination of the cellular mechanical environment. Here, we quantified vibration-induced fluid shear stresses in vitro and tested whether this system allows for the separation of two mechanical parameters previously proposed to drive the cellular response to vibration - fluid shear and peak accelerations. When peak accelerations of the oscillatory horizontal motions were set at 1g and 60Hz, peak fluid shear stresses acting on the cell layer reached 0.5Pa. A 3.5-fold increase in fluid viscosity increased peak fluid shear stresses 2.6-fold while doubling fluid volume in the well caused a 2-fold decrease in fluid shear. Fluid shear was positively related to peak acceleration magnitude and inversely related to vibration frequency. These data demonstrated that peak shear stress can be effectively separated from peak acceleration by controlling specific levels of vibration frequency, acceleration, and/or fluid viscosity. As an example for exploiting these relations, we tested the relevance of shear stress in promoting COX-2 expression in osteoblast like cells. Across different vibration frequencies and fluid viscosities, neither the level of generated fluid shear nor the frequency of the signal were able to consistently account for differences in the relative increase in COX-2 expression between groups, emphasizing that the eventual identification of the physical mechanism(s) requires a detailed quantification of the cellular mechanical environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.