ArticleNucleic acids research2013
Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Comprehensive evaluation of T7 promoter for enhanced yield and quality in mRNA production.Scientific reports · 2024Article
- Clonal Amplification-Enhanced Gene Expression in Synthetic Vesicles.ACS synthetic biology · 2023Article
- T7Max transcription system.Journal of biological engineering · 2023Article
- High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription.Bioresources and bioprocessing · 2022Article
- Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.Nucleic acids research · 2020Article
- Photocrosslinking of cDNA Display Molecules with Their Target Proteins as a New Strategy for Peptide Selection.Molecules (Basel, Switzerland) · 2020Article
- Synthetic Biology Goes Cell-Free.BMC biology · 2019Review
- Promoter RNA sequencing (PRSeq) for the massive and quantitative promoter analysis in vitro.Scientific reports · 2019Article
- Article
- Challenges of influenza A viruses in humans and animals and current animal vaccines as an effective control measure.Clinical and experimental vaccine research · 2018Review
- High-throughput screening of biomolecules using cell-free gene expression systems.Synthetic biology (Oxford, England) · 2018Review
- Chemoselective Coupling Preserves the Substrate Integrity of Surface-Immobilized Oligonucleotides for Emulsion PCR-Based Gene Library Construction.ACS combinatorial science · 2017Article
- An in vitro compartmentalization-based method for the selection of bond-forming enzymes from large libraries.Biotechnology and bioengineering · 2016Article
- A mix-and-read drop-based in vitro two-hybrid method for screening high-affinity peptide binders.Scientific reports · 2016Article
- Conceptual and methodological advances in cell-free directed evolution.Current opinion in structural biology · 2015Review
- Article
- Peptide aptamers: development and applications.Current topics in medicinal chemistry · 2015Review
- Advances in the directed evolution of proteins.Current opinion in chemical biology · 2014Review
- Linking genotype to phenotype on beads: high throughput selection of peptides with biological function.Scientific reports · 2013Article
- In vitro affinity screening of protein and peptide binders by megavalent bead surface display.Protein engineering, design & selection : PEDS · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro evolution of nucleic acids and proteins is a powerful strategy to optimize their biological and physical properties. To select proteins with the desired phenotype from large gene libraries, the proteins need to be linked to the gene they are encoded by. To facilitate selection of the desired phenotype and isolation of the encoding DNA, a novel bead display approach was developed, in which each member of a library of beads is first linked to multiple copies of a clonal gene variant by emulsion polymerase chain reaction. Beads are transferred to a second emulsion for an in vitro transcription-translation reaction, in which the protein encoded by each bead's amplicon covalently binds to the bead present in the same picoliter reactor. The beads then contain multiple copies of a clonal gene variant and multiple molecules of the protein encoded by the bead's gene variant and serve as the unit of selection. As a proof of concept, we screened a randomized library of the T7 promoter for high expression levels by flow cytometry and identified a T7 promoter variant with an ~10-fold higher in vitro transcriptional activity, confirming that the multi-copy bead display approach can be efficiently applied to in vitro evolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.