ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2013
In vitro oocyte culture-based manipulation of zebrafish maternal genes.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis.Cell reports · 2024Article
- Perfluorooctanoic Acid (PFOA) Exposure Compromises Fertility by Affecting Ovarian and Oocyte Development.International journal of molecular sciences · 2023Article
- Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes.PLoS biology · 2023Article
- Differential nuclear import sets the timing of protein access to the embryonic genome.Nature communications · 2022Article
- Transcriptomic Profiling Revealed Signaling Pathways Associated with the Spawning of Female Zebrafish under Cold Stress.International journal of molecular sciences · 2022Article
- Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants.Journal of visualized experiments : JoVE · 2021Article
- Zebrafish Ski7 tunes RNA levels during the oocyte-to-embryo transition.PLoS genetics · 2021Article
- Microinjection Method for Analyzing Zebrafish Early Stage Oocytes.Frontiers in cell and developmental biology · 2021Review
- Improving CRISPR/Cas9 mutagenesis efficiency by delaying the early development of zebrafish embryos.Scientific reports · 2020Article
- The Ly6/uPAR protein Bouncer is necessary and sufficient for species-specific fertilization.Science (New York, N.Y.) · 2018Article
- Methods for the analysis of early oogenesis in Zebrafish.Developmental biology · 2017Article
- Zebrafish lipid droplets regulate embryonic ATP homeostasis to power early development.Open biology · 2017Article
- Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish.Journal of visualized experiments : JoVE · 2017Article
- Myosin-1 inhibition by PClP affects membrane shape, cortical actin distribution and lipid droplet dynamics in early Zebrafish embryos.PloS one · 2017Article
- A novel technique based on in vitro oocyte injection to improve CRISPR/Cas9 gene editing in zebrafish.Scientific reports · 2016Article
- aura (mid1ip1l) regulates the cytoskeleton at the zebrafish egg-to-embryo transition.Development (Cambridge, England) · 2016Article
- Methods to study maternal regulation of germ cell specification in zebrafish.Methods in cell biology · 2016Article
- Zebrafish: a vertebrate tool for studying basal body biogenesis, structure, and function.Cilia · 2016Review
- Souffle/Spastizin controls secretory vesicle maturation during zebrafish oogenesis.PLoS genetics · 2014Article
- The chromosomal passenger protein birc5b organizes microfilaments and germ plasm in the zebrafish embryo.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In animals, females deposit gene products into developing oocytes, which drive early cellular events in embryos immediately after fertilization. As maternal gene products are present before fertilization, the functional manipulation of maternal genes is often challenging to implement, requiring gene expression or targeting during oogenesis. Maternal expression can be achieved through transgenesis, but transgenic approaches are time consuming and subject to undesired epigenetic effects. Here, we have implemented in vitro culturing of experimentally manipulated immature oocytes to study maternal gene contribution to early embryonic development in the zebrafish. We demonstrate phenotypic rescue of a maternal-effect mutation by expressing wild-type product in cultured oocytes. We also generate loss-of-function phenotypes in embryos through either the expression of a dominant-negative transcript or injection of translation-blocking morpholino oligonucleotides. Finally, we demonstrate subcellular localization during the early cell divisions immediately after fertilization of an exogenously provided maternal product fused to a fluorescent protein. These manipulations extend the potential to carry out genetic and imaging studies of zebrafish maternal genes during the egg-to-embryo transition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.