ArticleDrug metabolism and disposition: the biological fate of chemicals2013
Generation and characterization of a novel Cyp2a(4/5)bgs-null mouse model.
Article in Drug metabolism and disposition: the biological fate of chemicals, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Role of P450 enzymes of the Cyp2abfgs gene subfamilies in tobacco smoke-induced lung tumorigenesis in mice.Carcinogenesis · 2026Article
- The Role of Nicotine Metabolic Rate on Nicotine Dependence and Rewarding: Nicotine Metabolism in Chinese Male Smokers and Male Mice.Molecular neurobiology · 2024Article
- NLRP inflammasomes in health and disease.Molecular biomedicine · 2024Review
- Enantiomeric Fractions Reveal Differences in the Atropselective Disposition of 2,2',3,5',6-Pentachlorobiphenyl (PCB 95) in Wildtype,Chemical research in toxicology · 2023Article
- Probing the Role of CYP2 Enzymes in the Atropselective Metabolism of Polychlorinated Biphenyls Using Liver Microsomes from Transgenic Mouse Models.Chemical research in toxicology · 2022Article
- Human Orphan Cytochromes P450: An Update.Current drug metabolism · 2022Article
- Assessing cytochrome P450 function using genetically engineered mouse models.Advances in pharmacology (San Diego, Calif.) · 2022Review
- Analyzing the metabolic fate of oral administration drugs: A review and state-of-the-art roadmap.Frontiers in pharmacology · 2022Review
- NLRP9 in innate immunity and inflammation.Immunology · 2021Review
- Review
- Maternally contributed Nlrp9b expressed in human and mouse ovarian follicles contributes to early murine preimplantation development.Journal of assisted reproduction and genetics · 2020Article
- A Role for the Orphan Human Cytochrome P450 2S1 in Polyunsaturated Fatty AcidDrug metabolism and disposition: the biological fate of chemicals · 2019Article
- Single cell RNA sequencing of human liver reveals distinct intrahepatic macrophage populations.Nature communications · 2018Article
- Role of CYP2B in Phenobarbital-Induced Hepatocyte Proliferation in Mice.Drug metabolism and disposition: the biological fate of chemicals · 2017Article
- Compensatory changes in CYP expression in three different toxicology mouse models: CAR-null, Cyp3a-null, and Cyp2b9/10/13-null mice.PloS one · 2017Article
- Suppression of pulmonary CYP2A13 expression by carcinogen-induced lung tumorigenesis in a CYP2A13-humanized mouse model.Drug metabolism and disposition: the biological fate of chemicals · 2015Article
- NLRP9B protein is dispensable for oocyte maturation and early embryonic development in the mouse.The Journal of reproduction and development · 2015Article
- Article
- Effects of methoxsalen, a CYP2A5/6 inhibitor, on nicotine dependence behaviors in mice.Neuropharmacology · 2014Article
- Impact of nicotine metabolism on nicotine's pharmacological effects and behavioral responses: insights from a Cyp2a(4/5)bgs-null mouse.The Journal of pharmacology and experimental therapeutics · 2013Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Knockout mouse models targeting various cytochrome P450 (P450 or CYP) genes are valuable for determining P450's biologic functions, including roles in drug metabolism and chemical toxicity. In this study, a novel Cyp2a(4/5)bgs-null mouse model was generated, in which a 1.2-megabase pair genomic fragment containing nine Cyp genes in mouse chromosome 7 (including, sequentially, Cyp2a5, 2g1, 2b19, 2b23, 2a4, 2b9, 2b13, 2b10, and 2s1) are deleted, through Cre-mediated recombination in vivo. The resultant mouse strain was viable and fertile, without any developmental deficits or morphologic abnormalities. Deletion of the constitutive genes in the cluster was confirmed by polymerase chain reaction analysis of the genes and the mRNAs in tissues known to express each gene. The loss of this gene cluster led to significant decreases in microsomal activities toward testosterone hydroxylation in various tissues examined, including olfactory mucosa (OM), lung, liver, and brain. In addition, systemic clearance of pentobarbital was decreased in Cyp2a(4/5)bgs-null mice, as indicated by >60% increases in pentobarbital-induced sleeping time, compared with wild-type (WT) mice. This novel Cyp2a(4/5)bgs-null mouse model will be valuable for in vivo studies of drug metabolism and chemical toxicities in various tissues, including the liver, lung, brain, intestine, kidney, skin, and OM, where one or more of the targeted Cyp genes are known to be expressed in WT mice. The model will also be valuable for preparation of humanized mice that express human CYP2A6, CYP2A13, CYP2B6, or CYP2S1, and as a knockout mouse model for five non-P450 genes (Vmn1r184, Nalp9c, Nalp4a, Nalp9a, and Vmn1r185) that were also deleted.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.