ReviewBone2013
In vitro and in vivo approaches to study osteocyte biology.
Review in Bone, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
87 citing papers in PubMed, 151 citations in OpenAlex.
- DMP1-Cre expressing cells mediate the gain in bone mass and strength, but not the increase in bone remodeling, induced by ligands of the parathyroid hormone receptor.Bone research · 2026Article
- Region- and Compartment-Specific Elevation of Bone Mass in Mice Following Tsc1 Deletion in 8-kb Dmp1-Cre-Expressing Cells.Calcified tissue international · 2026Article
- Bone Organoids: A Novel Tool for Modeling and Managing Skeletal Disorders in Diabetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- New knock-in mouse linesJBMR plus · 2026Article
- Generation and characterization of a novel inducible Sost_P2A_CreERT2 mouse model with high specificity for osteocytes.JBMR plus · 2026Article
- Mechanical Cues and Lineage Commitment Govern the Angiogenic Potential of Mesenchymal Cell-Derived Extracellular Vesicles.Advanced biology · 2026Article
- The development of a collagen-nanoscale hydroxyapatite three-dimensional (3D) in vitro culture system for reproducing osteocyte differentiation and tissue mineralization.Biomaterials · 2026Article
- Angiotensin II Promotes Osteocyte RANKL Expression via AT1R Activation.Biomedicines · 2025Article
- Osteocyte gene expression analysis in mouse bone: optimization of a laser-assisted microdissection protocol.JBMR plus · 2024Article
- Integrating Mechanics and Bioactivity: A Detailed Assessment of Elasticity and Viscoelasticity at Different Scales in 2D Biofunctionalized PEGDA Hydrogels for Targeted Bone Regeneration.ACS applied materials & interfaces · 2024Article
- High-fat and high-carbohydrate diets increase bone fragility through TGF-β-dependent control of osteocyte function.JCI insight · 2024Article
- Hematopoietic and stromal DMP1-Cre labeled cells form a unique niche in the bone marrow.Scientific reports · 2023Article
- A framework for defining mesenchymal cell types associated with murine periosteal and endosteal bone.bioRxiv : the preprint server for biology · 2023Article
- Differing responses of osteogenic cell lines to β-glycerophosphate.Scientific reports · 2023Article
- CRISPR interference provides increased cell type-specificity compared to the Cre-loxP system.iScience · 2023Article
- Why Animal Experiments Are Still Indispensable in Bone Research: A Statement by the European Calcified Tissue Society.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Review
- Generation of bicistronic Dmp1-Cre knock-in mice using a self-cleaving 2A peptide.Journal of bone and mineral metabolism · 2023Article
- Targeting osteocytes vs osteoblasts.Bone · 2023Review
- Review
- Local senolysis in aged mice only partially replicates the benefits of systemic senolysis.The Journal of clinical investigation · 2023Article
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Osteocytes, the most abundant cell population of the bone lineage, have been a major focus in the bone research field in recent years. This population of cells that resides within mineralized matrix is now thought to be the mechanosensory cell in bone and plays major roles in the regulation of bone formation and resorption. Studies of osteocytes had been impaired by their location, resulting in numerous attempts to isolate primary osteocytes and to generate cell lines representative of the osteocytic phenotype. Progress has been achieved in recent years by utilizing in vivo genetic technology and generation of osteocyte directed transgenic and gene deficiency mouse models. We will provide an overview of the current in vitro and in vivo models utilized to study osteocyte biology. We discuss generation of osteocyte-like cell lines and isolation of primary osteocytes and summarize studies that have utilized these cellular models to understand the functional role of osteocytes. Approaches that attempt to selectively identify and isolate osteocytes using fluorescent protein reporters driven by regulatory elements of genes that are highly expressed in osteocytes will be discussed. In addition, recent in vivo studies utilizing overexpression or conditional deletion of various genes using dentin matrix protein (Dmp1) directed Cre recombinase are outlined. In conclusion, evaluation of the benefits and deficiencies of currently used cell lines/genetic models in understanding osteocyte biology underlines the current progress in this field. The future efforts will be directed towards developing novel in vitro and in vivo models that would additionally facilitate in understanding the multiple roles of osteocytes.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.