ArticleTissue engineering. Part A2013
Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogel.
Article in Tissue engineering. Part A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 2 of them syntheses that pooled it.
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Who cites it
67 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Pooled it
- NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review.Theranostics · 2020Pooled it
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- Engineering Extracellular Vesicle Production Through Magnetic Ion Channel Activation for Bone Regeneration.Advanced healthcare materials · 2026Article
- Bone morphogenetic protein-2-derived osteogenic peptide promotes bone regeneration via osteoblastogenesis.Regenerative therapy · 2025Article
- Systemic Effect of Thermo-Photobiomodulation on Mesenchymal Stem Cells.Bulletin of experimental biology and medicine · 2025Article
- Tauroursodeoxycholic acid-induced increase in ectopic muscle mineralization occurs exclusively in dystrophic muscles and is independent of endoplasmic reticulum stress.Scientific reports · 2025Article
- Multifunctional Hydrogel with Synergistic Reactive Oxygen Species Scavenging and Macrophage Polarization-Induced Osteo-immunomodulation for Enhanced Bone Regeneration.ACS applied materials & interfaces · 2025Article
- Low-Temperature Spine-Specific PMMA Enhances Bone Regeneration via Localized Thermal Necrosis in an Osteoporotic Rat Model.International journal of molecular sciences · 2025Article
- Heat Shock Strengthens the Protective Potential of MSCs in Liver Injury by Promoting EV Release Through Upregulated Autophagosome Formation.Journal of extracellular vesicles · 2025Article
- Photothermal Coating on Zinc Alloy for Controlled Biodegradation and Improved Osseointegration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Synergistic effects of biological stimuli and flexion induce microcavities promote hypertrophy and inhibit chondrogenesis during in vitro culture of human mesenchymal stem cell aggregates.Biotechnology journal · 2024Article
- Photoacoustic Imaging-Guided Self-Adaptive Hyperthermia Supramolecular Cascade Nano-Reactor for Diabetic Periodontal Bone Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Photothermal driven BMSCs osteogenesis and M2 macrophage polarization on polydopamine-coated TiMaterials today. Bio · 2024Article
- Analysis of theCells · 2024Article
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- Near-infrared light responsive gold nanoparticles coating endows polyetheretherketone with enhanced osseointegration and antibacterial properties.Materials today. Bio · 2024Article
- Effect of an α-calcium sulfate hemihydrate/treated dentin matrix composite to regenerate bone in critically sized SD rat calvarial defects.Frontiers in bioengineering and biotechnology · 2024Article
- Synthesis and characterization of a magnetic bacterial cellulose-chitosan nanocomposite and evaluation of its applicability for osteogenesis.BioImpacts : BI · 2024Article
7 more citing papers are in PubMed but not listed here.
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Funding
Abstract
The mechanisms for the heat-induced osteogenesis are not completely known and the thermal regulation of human mesenchymal stem cell (hMSC) differentiation is not well studied. In this study, the direct effects of mild heat shock (HS) on the differentiation of hMSCs into osteoblasts in self-assembling peptide hydrogel and on tissue culture plates were investigated. hMSCs isolated from human bone marrow were seeded in conventional culture plates (two-dimensional [2D] culture) and on the surface of three-dimensional (3D) PuraMatrix peptide hydrogel (3D culture), followed by 1 h HS at 41°C once a week during osteogenic differentiation. Alkaline phosphatase (ALP) activity was enhanced in both 2D and 3D cultures via periodic HS at early stage of differentiation; meanwhile, HS significantly increased the calcium deposition at day 19 and 27 of differentiation in both 2D and 3D cultures. The periodic HS also upregulated osteo-specific genes, osterix (OSX) on day 11, osteopontin (OP) on day 19, and bone morphogenetic protein 2 (BMP2) on day 25 in 2D culture. In 3D PuraMatrix culture, the runt-related transcription factor 2 (Runx2) was upregulated by HS on day 25 of differentiation. The heat shock protein 70 (HSP70) was significantly upregulated by HS in differentiated hMSCs analyzed at 24 h after HS. These results demonstrate that HS induced an earlier differentiation of hMSCs and enhanced the maturation of osteoblasts differentiated from hMSCs. Therefore, mild HS treatment may be potentially used to enhance the bone regeneration using hMSCs. Our data will guide the design of in vivo heating protocols and enable further investigations in thermal treatments of MSC osteogenesis for bone tissue engineering.
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