ArticlePloS one2012
Knockout of multiple Arabidopsis cation/H(+) exchangers suggests isoform-specific roles in metal stress response, germination and seed mineral nutrition.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- Genome-wide identification of long non-coding RNAs revealing their regulatory roles under seed development in ricebean (Vigna umbellata).BMC plant biology · 2026Article
- Genome-wide association study of alkali tolerance at germination stage in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- CAX1 beyond calcium transport: evidence for a transport-independent regulatory module in plants.Frontiers in plant science · 2026Review
- Time-Series Elemental Imaging Reveals CAX-Dependent Redistribution Patterns for Anoxia Recovery.Journal of experimental botany · 2025Article
- Genome-Wide Identification ofGenes · 2023Article
- Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis inPlant direct · 2023Article
- A dual-flow RootChip enables quantification of bi-directional calcium signaling in primary roots.Frontiers in plant science · 2022Article
- Transport, functions, and interaction of calcium and manganese in plant organellar compartments.Plant physiology · 2021Review
- A curated list of genes that affect the plant ionome.Plant direct · 2020Article
- Manganese in Plants: From Acquisition to Subcellular Allocation.Frontiers in plant science · 2020Review
- Advances in the Mechanisms of Plant Tolerance to Manganese Toxicity.International journal of molecular sciences · 2019Review
- Nitrogen Starvation Differentially Influences Transcriptional and Uptake Rate Profiles in Roots of Two Maize Inbred Lines with Different NUE.International journal of molecular sciences · 2019Article
- Overexpression of a High-Affinity Nitrate TransporterFrontiers in plant science · 2018Article
- Heterodimerization of Arabidopsis calcium/proton exchangers contributes to regulation of guard cell dynamics and plant defense responses.Journal of experimental botany · 2017Article
- CAX-ing a wide net: Cation/H(+) transporters in metal remediation and abiotic stress signalling.Plant biology (Stuttgart, Germany) · 2016Review
- Calcium-Mediated Abiotic Stress Signaling in Roots.Frontiers in plant science · 2016Review
- Mn-euvering manganese: the role of transporter gene family members in manganese uptake and mobilization in plants.Frontiers in plant science · 2014Review
- Calcium Signals from the Vacuole.Plants (Basel, Switzerland) · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Cation/H(+) exchangers encoded by CAX genes play an important role in the vacuolar accumulation of metals including Ca(2+) and Mn(2+). Arabidopsis thaliana CAX1 and CAX3 have been previously shown to differ phylogenetically from CAX2 but the physiological roles of these different transporters are still unclear. To examine the functions and the potential of redundancy between these three cation transporters, cax1/cax2 and cax2/cax3 double knockout mutants were generated and compared with wild type and cax single knockouts. These double mutants had equivalent metal stress responses to single cax mutants. Both cax1 and cax1/cax2 had increased tolerance to Mg stress, while cax2 and cax2/cax3 both had increased sensitivity to Mn stress. The cax1/cax2 and cax2/cax3 mutants did not exhibit the deleterious developmental phenotypes previously seen with the cax1/cax3 mutant. However, these new double mutants did show alterations in seed germination, specifically a delay in germination time. These alterations correlated with changes in nutrient content within the seeds of the mutants, particularly the cax1/cax2 mutant which had significantly higher seed content of Ca and Mn. This study indicates that the presence of these Arabidopsis CAX transporters is important for normal germination and infers a role for CAX proteins in metal homeostasis within the seed.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.