ArticlePloS one2012
Targeted genomic integration of a selectable floxed dual fluorescence reporter in human embryonic stem cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Bidirectional fibrogenic cross-talk revealed in a human iPSC-derived epithelial-mesenchymal co-culture model of pulmonary fibrosis.bioRxiv : the preprint server for biology · 2026Article
- Stacking thick perfusable human microvascular grafts enables dense vascularity and rapid integration into infarcted rat hearts.Biomaterials · 2023Article
- Progress in the production of haematopoietic stem and progenitor cells from human pluripotent stem cells.Journal of immunology and regenerative medicine · 2021Article
- Molecular Signatures and Networks of Cardiomyocyte Differentiation in Humans and Mice.Molecular therapy. Nucleic acids · 2020Article
- A Rainbow Reporter Tracks Single Cells and Reveals Heterogeneous Cellular Dynamics among Pluripotent Stem Cells and Their Differentiated Derivatives.Stem cell reports · 2020Article
- The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming.Cell reports · 2019Article
- Patterned human microvascular grafts enable rapid vascularization and increase perfusion in infarcted rat hearts.Nature communications · 2019Article
- Induced Pluripotent Stem Cells for Cardiovascular Disease Modeling and Precision Medicine: A Scientific Statement From the American Heart Association.Circulation. Genomic and precision medicine · 2018Review
- Inducible CRISPR genome editing platform in naive human embryonic stem cells reveals JARID2 function in self-renewal.Cell cycle (Georgetown, Tex.) · 2018Article
- CRISPR/Cas9-loxP-Mediated Gene Editing as a Novel Site-Specific Genetic Manipulation Tool.Molecular therapy. Nucleic acids · 2017Article
- Genome editing in cardiovascular diseases.Nature reviews. Cardiology · 2017Review
- Generating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells.Nature protocols · 2017Article
- Mitochondrial Maturation in Human Pluripotent Stem Cell Derived Cardiomyocytes.Stem cells international · 2017Article
- Site-Specific Genome Engineering in Human Pluripotent Stem Cells.International journal of molecular sciences · 2016Review
- Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development.Genome biology · 2015Article
- Cardiac development in zebrafish and human embryonic stem cells is inhibited by exposure to tobacco cigarettes and e-cigarettes.PloS one · 2015Article
- Cardiac tissue engineering and regeneration using cell-based therapy.Stem cells and cloning : advances and applications · 2015Review
- Pluripotent stem cell derived cardiomyocytes for cardiac repair.Current treatment options in cardiovascular medicine · 2014Article
- Article
- The roles of PDGF in development and during neurogenesis in the normal and diseased nervous system.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The differentiation of pluripotent stem cells involves transition through a series of specific cell states. To understand these cell fate decisions, the field needs improved genetic tools for the labeling, lineage tracing and selection of specific cell types from heterogeneous differentiating populations, particularly in the human embryonic stem cell (hESC) system. We used zinc finger nuclease technology to stably insert a unique, selectable, floxed dual-fluorescence reporter transgene into the AAVS1 locus of RUES2 hESCs. This "stoplight" transgene, mTmG-2a-Puro, strongly expresses membrane-localized tdTomato red fluorescent protein until Cre-dependent recombination causes a switch to expression of membrane-localized enhanced green fluorescent protein (eGFP) and puromycin resistance. First, to validate this system in undifferentiated cells, we transduced transgenic hESCs with a lentiviral vector driving constitutive expression of Cre and observed the expected phenotypic switch. Next, to demonstrate its utility in lineage-specific selection, we transduced differentiated cultures with a lentiviral vector in which the striated muscle-specific CK7 promoter drives Cre expression. This yielded near-homogenous populations of eGFP(+) hESC-derived cardiomyocytes. The mTmg-2a-Puro hESC line described here represents a useful new tool for both in vitro fate mapping studies and the selection of useful differentiated cell types.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.