ArticlePloS one2012
In vivo tumorigenesis was observed after injection of in vitro expanded neural crest stem cells isolated from adult bone marrow.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Monocyte chemoattractant protein-1 from a conditioned medium of bone marrow-derived mesenchymal stem cells promotes bone regeneration by enhancing macrophage phenotype polarization.Maxillofacial plastic and reconstructive surgery · 2026Article
- Histologic and Histomorphometric Evaluation of Bone Regeneration Using Human Allogeneic Bone Graft with or Without Mesenchymal Stem Cell-Conditioned Media in a Rabbit Calvarial Defect Model.Journal of functional biomaterials · 2025Article
- Review
- A new frontier in temporomandibular joint osteoarthritis treatment: Exosome-based therapeutic strategy.Frontiers in bioengineering and biotechnology · 2022Review
- Neural crest-like stem cells for tissue regeneration.Stem cells translational medicine · 2021Review
- Article
- Article
- Correction: Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues.PloS one · 2021Article
- Sanhua Decoction, a Classic Herbal Prescription, Exerts Neuroprotection Through Regulating Phosphorylated Tau Level and Promoting Adult Endogenous Neurogenesis After Cerebral Ischemia/Reperfusion Injury.Frontiers in physiology · 2020Article
- Tumor Formation of Adult Stem Cell Transplants in Rodent Arthritic Joints.Molecular imaging and biology · 2019Article
- Article
- A defined mix of cytokines mimics conditioned medium from cultures of bone marrow-derived mesenchymal stem cells and elicits bone regeneration.Cell proliferation · 2017Article
- Analgesia for neuropathic pain by dorsal root ganglion transplantation of genetically engineered mesenchymal stem cells: initial results.Molecular pain · 2015Article
- Role of mesenchymal stem cells in bone regeneration and fracture repair: a review.International orthopaedics · 2013Review
- Effects of valproic acid on histone deacetylase inhibition in vitro and in glioblastoma patient samples.Neuro-oncology advancesArticle
- Molecular characterization of heterogeneous mesenchymal stem cells with single-cell transcriptomes.Biotechnology advancesReview
Corrections and comments
- Erratum issued
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bone marrow stromal cells are adult multipotent cells that represent an attractive tool in cellular therapy strategies. Several studies have reported that in vitro passaging of mesenchymal stem cells alters the functional and biological properties of those cells, leading to the accumulation of genetic aberrations. Recent studies described bone marrow stromal cells (BMSC) as mixed populations of cells including mesenchymal (MSC) and neural crest stem cells (NCSC). Here, we report the transformation of NCSC into tumorigenic cells, after in vitro long-term passaging. Indeed, the characterization of 6 neural crest-derived clones revealed the presence of one tumorigenic clone. Transcriptomic analyses of this clone highlighted, among others, numerous cell cycle checkpoint modifications and chromosome 11q down-regulation (suggesting a deletion of chromosome 11q) compared with the other clones. Moreover, unsupervised analysis such as a dendrogram generated after agglomerative hierarchical clustering comparing several transcriptomic data showed important similarities between the tumorigenic neural crest-derived clone and mammary tumor cell lines. Altogether, it appeared that NCSC isolated from adult bone marrow represents a potential danger for cellular therapy, and consequently, we recommend that phenotypic, functional and genetic assays should be performed on bone marrow mesenchymal and neural crest stem cells before in vivo use, to demonstrate whether their biological properties, after ex vivo expansion, remain suitable for clinical application.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.