ArticlePloS one2012
Visualization of cortical projection neurons with retrograde TET-off lentiviral vector.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Efficient Labeling of Somatodendritic Membranes of the Central Neurons.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Visualization of Selective Neural Pathways by Double Viral Infection Method.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Tracing of specific neural pathways in the rat brain using lentiviral vectors for retrograde gene transfer.Anatomical science international · 2025Article
- Techniques to Render Dendritic Spines Visible in the Microscope.Advances in neurobiology · 2023Article
- Interstitial Axon Collaterals of Callosal Neurons Form Association Projections from the Primary Somatosensory to Motor Cortex in Mice.Cerebral cortex (New York, N.Y. : 1991) · 2021Article
- Adeno-Associated Virus Technologies and Methods for Targeted Neuronal Manipulation.Frontiers in neuroanatomy · 2019Review
- Development of lentiviral vectors for efficient glutamatergic-selective gene expression in cultured hippocampal neurons.Scientific reports · 2018Article
- Retrogradely Transportable Lentivirus Tracers for Mapping Spinal Cord Locomotor Circuits.Frontiers in neural circuits · 2018Article
- Application of viral vectors to the study of neural connectivities and neural circuits in the marmoset brain.Developmental neurobiology · 2017Review
- Article
- Neurophotonics applications to motor cortex research.Neurophotonics · 2014Article
- Simultaneous visualization of extrinsic and intrinsic axon collaterals in Golgi-like detail for mouse corticothalamic and corticocortical cells: a double viral infection method.Frontiers in neural circuits · 2014Article
- Monocular inhibition reveals temporal and spatial changes in gene expression in the primary visual cortex of marmoset.Frontiers in neural circuits · 2013Article
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Authors and funding
14 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We are interested in identifying and characterizing various projection neurons that constitute the neocortical circuit. For this purpose, we developed a novel lentiviral vector that carries the tetracycline transactivator (tTA) and the transgene under the TET Responsive Element promoter (TRE) on a single backbone. By pseudotyping such a vector with modified rabies G-protein, we were able to express palmitoylated-GFP (palGFP) or turboFP635 (RFP) in corticothalamic, corticocortical, and corticopontine neurons of mice. The high-level expression of the transgene achieved by the TET-Off system enabled us to observe characteristic elaboration of neuronal processes for each cell type. At higher magnification, we were able to observe fine structures such as boutons and spines as well. We also injected our retrograde TET-Off vector to the marmoset cortex and proved that it can be used to label the long-distance cortical connectivity of millimeter scale. In conclusion, our novel retrograde tracer provides an attractive option to investigate the morphologies of identified cortical projection neurons of various species.
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