ArticleProceedings of the National Academy of Sciences of the United States of America2012
Effects of long DNA folding and small RNA stem-loop in thermophoresis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Ludwig-Soret microscopy with the vibrational photothermal effect.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- Metastable phase-separated droplet generation and long-time DNA enrichment by laser-induced Soret effect.Communications chemistry · 2025Article
- Application of Resonant Plasmonic Bowtie Nanoantennas for Optically-Assisted Diffusiophoretic Trapping of Extracellular Vesicles and Nanoparticles.Laser & photonics reviews · 2025Article
- Thermophilic Behavior of Heat-Dissociative Coacervate Droplets.Nano letters · 2024Article
- CRISPR-powered optothermal nanotweezers: Diverse bio-nanoparticle manipulation and single nucleotide identification.Light, science & applications · 2023Article
- Non-covalent dyes in microscale thermophoresis for studying RNA ligand interactions and modifications.Chemical science · 2023Article
- Multimodal Optothermal Manipulations along Various Surfaces.ACS nano · 2023Article
- Optothermal rotation of micro-/nano-objects.Chemical communications (Cambridge, England) · 2023Review
- Article
- Article
- Programmable Multimodal Optothermal Manipulation of Synthetic Particles and Biological Cells.ACS nano · 2022Article
- Universal optothermal micro/nanoscale rotors.Science advances · 2022Article
- The role of temperature-induced effects generated by plasmonic nanostructures on particle delivery and manipulation: a review.Nanophotonics (Berlin, Germany) · 2022Review
- Heat-Mediated Optical Manipulation.Chemical reviews · 2022Review
- Opto-thermal diffusiophoresis of soft biological matter: from physical principle to molecular manipulation.Biophysical reviews · 2020Review
- Biophysics at Kyushu University.Biophysical reviews · 2020Article
- Plasmonic Manipulation of DNA using a Combination of Optical and Thermophoretic Forces: Separation of Different-Sized DNA from Mixture Solution.Scientific reports · 2020Article
- Thermal orientation and thermophoresis of anisotropic colloids: The role of the internal composition.The European physical journal. E, Soft matter · 2019Article
- Separation of Nano- and Microparticle Flows Using Thermophoresis in Branched Microfluidic Channels.Micromachines · 2019Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In thermophoresis, with the fluid at rest, suspensions move along a gradient of temperature. In an aqueous solution, a PEG polymer suspension is depleted from the hot region and builds a concentration gradient. In this gradient, DNA polymers of different sizes can be separated. In this work the effect of the polymer structure for genomic DNA and small RNA is studied. For genome-size DNA, individual single T4 DNA is visualized and tracked in a PEG solution under a temperature gradient built by infrared laser focusing. We find that T4 DNA follows steps of depletion, ring-like localization, and accumulation patterns as the PEG volume fraction is increased. Furthermore, a coil-globule transition for DNA is observed for a large enough PEG volume fraction. This drastically affects the localization position of T4 DNA. In a similar experiment, with small RNA such as ribozymes we find that the stem-loop folding of such polymers has important consequences. The RNA polymers having a long and rigid stem accumulate, whereas a polymer with stem length less than 4 base pairs shows depletion. Such measurements emphasize the crucial contribution of the double-stranded parts of RNA for thermal separation and selection under a temperature gradient. Because huge temperature gradients are present around hydrothermal vents in the deep ocean seafloor, this process might be relevant, at the origin of life, in an RNA world hypothesis. Ribozymes could be selected from a pool of random sequences depending on the length of their stems.
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