ArticleProceedings of the National Academy of Sciences of the United States of America2012
Controlled biosynthesis of odd-chain fuels and chemicals via engineered modular metabolic pathways.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 115 citations in OpenAlex.
- Recent advances in microbial production of odd-chain fatty acids.World journal of microbiology & biotechnology · 2026Review
- Relative Activities of the β-ketoacyl-CoA and Acyl-CoA Reductases Influence Product Profile and Flux in a Reversed β-Oxidation Pathway.ACS catalysis · 2023Article
- Enhancing the Production of Pinene inBiology · 2022Article
- Reverse β-oxidation pathways for efficient chemical production.Journal of industrial microbiology & biotechnology · 2022Article
- Implementation of Synthetic Pathways to Foster Microbe-Based Production of Non-Naturally Occurring Carboxylic Acids and Derivatives.Journal of fungi (Basel, Switzerland) · 2021Review
- Engineering precursor pools for increasing production of odd-chain fatty acids inMetabolic engineering communications · 2021Article
- Engineering nature for gaseous hydrocarbon production.Microbial cell factories · 2020Review
- Microbial synthesis of functional odd-chain fatty acids: a review.World journal of microbiology & biotechnology · 2020Review
- A novel C-terminal degron identified in bacterial aldehyde decarbonylases using directed evolution.Biotechnology for biofuels · 2020Article
- An Efficient New Process for the Selective Production of Odd-Chain Carboxylic Acids by Simple Carbon Elongation Using Megasphaera hexanoica.Scientific reports · 2019Article
- Engineering the pathway in Escherichia coli for the synthesis of medium-chain-length polyhydroxyalkanoates consisting of both even- and odd-chain monomers.Microbial cell factories · 2019Article
- Leveraging synthetic biology for producing bioactive polyketides and non-ribosomal peptides in bacterial heterologous hosts.MedChemComm · 2019Review
- Physiological, Genetic, and Transcriptomic Analysis of Alcohol-Induced Delay ofApplied and environmental microbiology · 2019Article
- Article
- Metabolic engineering to enhance biosynthesis of both docosahexaenoic acid and odd-chain fatty acids inBiotechnology for biofuels · 2019Article
- A seamless and iterative DNA assembly method named PS-Brick and its assisted metabolic engineering for threonine and 1-propanol production.Biotechnology for biofuels · 2019Article
- Microbial biosynthesis of lactate esters.Biotechnology for biofuels · 2019Article
- Rational design of thiolase substrate specificity for metabolic engineering applications.Biotechnology and bioengineering · 2018Article
- Saturated mutagenesis of ketoisovalerate decarboxylase V461 enabled specific synthesis of 1-pentanol via the ketoacid elongation cycle.Scientific reports · 2017Article
- Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid.Chemical science · 2017Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial systems are being increasingly developed as production hosts for a wide variety of chemical compounds. Broader adoption of microbial synthesis is hampered by a limited number of high-yielding natural pathways for molecules with the desired physical properties, as well as the difficulty in functionally assembling complex biosynthetic pathways in heterologous hosts. Here, we address both of these challenges by reporting the adaptation of the butanol biosynthetic pathway for the synthesis of odd-chain molecules and the development of a complementary modular toolkit to facilitate pathway construction, characterization, and optimization in engineered Escherichia coli. The modular feature of our pathway enables multientry and multiexit biosynthesis of various odd-chain compounds at high efficiency. By varying combinations of the pathway and toolkit enzymes, we demonstrate controlled production of propionate, trans-2-pentenoate, valerate, and pentanol, compounds with applications that include biofuels, antibiotics, biopolymers, and aroma chemicals. Importantly, and in contrast to a previously used method to identify limitations in heterologous amorphadiene production, our bypass strategy was effective even without the presence of freely membrane-diffusible substrates. This approach should prove useful for optimization of other pathways that use CoA-derivatized intermediates, including fatty acid β-oxidation and the mevalonate pathway for isoprenoid synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.