ArticleMolecular biology reports2012
Development of a broad-spectrum fluorescent heavy metal bacterial biosensor.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Duckweed: a potential phytosensor for heavy metals.Plant cell reports · 2022Review
- The protein scaffold calibrates metal specificity and activation in MerR sensors.Microbial biotechnology · 2022Article
- Detection of environmental pollutant cadmium in water using a visual bacterial biosensor.Scientific reports · 2022Article
- Recent advances in bacterial biosensing and bioremediation of cadmium pollution: a mini-review.World journal of microbiology & biotechnology · 2021Review
- Live-Cell Systems in Real-Time Biomonitoring of Water Pollution: Practical Considerations and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- In-situ monitoring of xenobiotics using genetically engineered whole-cell-based microbial biosensors: recent advances and outlook.World journal of microbiology & biotechnology · 2021Review
- Detection of Bioavailable Cadmium by Double-Color Fluorescence Based on a Dual-Sensing Bioreporter System.Frontiers in microbiology · 2021Article
- Modulation of the Metal(loid) Specificity of Whole-Cell Bioreporters by Genetic Engineering of ZntR Metal-Binding Loops.Journal of microbiology and biotechnology · 2020Article
- A Single-Layer PDMS Chamber for On-Chip Bacteria Culture.Micromachines · 2020Article
- Using the promoters of MerR family proteins as "rheostats" to engineer whole-cell heavy metal biosensors with adjustable sensitivity.Journal of biological engineering · 2019Article
- Use of Tunable Whole-Cell Bioreporters to Assess Bioavailable Cadmium and Remediation Performance in Soils.PloS one · 2016Article
- Precise metabolic engineering of carotenoid biosynthesis in Escherichia coli towards a low-cost biosensor.Metabolic engineering · 2015Article
- Determination of zinc, cadmium and lead bioavailability in contaminated soils at the single-cell level by a combination of whole-cell biosensors and flow cytometry.Sensors (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial biosensors can measure pollution in terms of their actual toxicity to living organisms. A recombinant bacterial biosensor has been constructed that is known to respond to toxic levels of Zn(2+), Cd(2+) and Hg(2+). The zinc regulatory gene zntR and zntA promoter from znt operon of E. coli have been used to trigger the expression of GFP reporter protein at toxic levels of these ions. The sensor was induced with 3-800 ppm of Zn(2+), 0.005-4 ppm of Cd(2+) and 0.001-0.12 ppm of Hg(2+) ions. Induction studies were also performed in liquid media to quantify GFP fluorescence using fluorimeter. To determine the optimum culture conditions three different incubation periods (16, 20 and 24 h) were followed. Results showed an increased and consistent fluorescence in cells incubated for 16 h. Maximum induction for Zn(2+), Cd(2+) and Hg(2+) was observed at 20, 0.005 and 0.002 ppm, respectively. The pPROBE-zntR-zntA biosensor reported here can be employed as a primary screening technique for aquatic heavy metal pollution.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.