ArticleThe Journal of physiology2013
Evaluation of glutamate concentration transient in the synaptic cleft of the rat calyx of Held.
Article in The Journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Distinct transmission sites within a synapse for strengthening and homeostasis.Science advances · 2025Article
- Article
- Cellular and synaptic specializations for sub-millisecond precision in the mammalian auditory brainstem.Frontiers in cellular neuroscience · 2025Review
- The role of responsive MRI probes in the past and the future of molecular imaging.Chemical science · 2024Review
- Cortico-striatal action control inherent of opponent cognitive-motivational styles.bioRxiv : the preprint server for biology · 2024Article
- The impact of diffusion on receptor binding during synaptic transmission.Biophysical journal · 2024Article
- Fusion pore flux controls the rise-times of quantal synaptic responses.The Journal of general physiology · 2024Article
- Rescuing tri-heteromeric NMDA receptor function: the potential of pregnenolone-sulfate in loss-of-function GRIN2B variants.Cellular and molecular life sciences : CMLS · 2024Article
- Glutamate Provides Cytoprotective Effect for Astrocytes Against Ischemic Insult and Promotes Astrogliosis.Aging and disease · 2024Article
- Maintenance of a central high frequency synapse in the absence of synaptic activity.Frontiers in cellular neuroscience · 2024Article
- Laying the foundations for a theory of consciousness: the significance of critical brain dynamics for the formation of conscious states.Frontiers in human neuroscience · 2024Article
- High calcium concentrations reduce cellular excitability of mouse MNTB neurons.Brain research · 2023Article
- Group I metabotropic glutamate receptor-triggered temporally patterned action potential-dependent spontaneous synaptic transmission in mouse MNTB neurons.Hearing research · 2023Article
- Afferent convergence to a shared population of interneuron AMPA receptors.Nature communications · 2023Article
- Optical analysis of glutamate spread in the neuropil.Cerebral cortex (New York, N.Y. : 1991) · 2022Article
- Membrane Stretch Gates NMDA Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Neuroligin-3 confines AMPA receptors into nanoclusters, thereby controlling synaptic strength at the calyx of Held synapses.Science advances · 2022Article
- Computational modeling of trans-synaptic nanocolumns, a modulator of synaptic transmission.Frontiers in computational neuroscience · 2022Article
- Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images.Frontiers in neuroanatomy · 2022Article
- Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.Pharmacological reviews · 2021Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Establishing the spatiotemporal concentration profile of neurotransmitter following synaptic vesicular release is essential for our understanding of inter-neuronal communication. Such profile is a determinant of synaptic strength, short-term plasticity and inter-synaptic crosstalk. Synaptically released glutamate has been suggested to reach a few millimolar in concentration and last for <1 ms. The synaptic cleft is often conceived as a single concentration compartment, whereas a huge gradient likely exists. Modelling studies have attempted to describe this gradient, but two key parameters, the number of glutamate in a vesicle (N(Glu)) and its diffusion coefficient (D(Glu)) in the extracellular space, remained unresolved. To determine this profile, the rat calyx of Held synapse at postnatal day 12-16 was studied where diffusion of glutamate occurs two-dimensionally and where quantification of AMPA receptor distribution on individual postsynaptic specialization on medial nucleus of the trapezoid body principal cells is possible using SDS-digested freeze-fracture replica labelling. To assess the performance of these receptors as glutamate sensors, a kinetic model of the receptors was constructed from outside-out patch recordings. From here, we simulated synaptic responses and compared them with the EPSC recordings. Combinations of N(Glu) and D(Glu) with an optimum of 7000 and 0.3 μm(2) ms(-1) reproduced the data, suggesting slow diffusion. Further simulations showed that a single vesicle does not saturate the synaptic receptors, and that glutamate spillover does not affect the conductance amplitude at this synapse. Using the estimated profile, we also evaluated how the number of multiple vesicle releases at individual active zones affects the amplitude of postsynaptic signals.
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