ArticleAnalytical biochemistry2013
Quantification of neutral human milk oligosaccharides by graphitic carbon high-performance liquid chromatography with tandem mass spectrometry.
Article in Analytical biochemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
43 citing papers in PubMed, 2 syntheses or guidelines pooled it, 118 citations in OpenAlex.
- Systematic review of the concentrations of oligosaccharides in human milk.Nutrition reviews · 2017Pooled it
- A systematic review and meta-analysis of the nutrient content of preterm and term breast milk.BMC pediatrics · 2014Pooled it
- An Extensively Hydrolyzed Formula Supplemented with Two Human Milk Oligosaccharides Modifies the Fecal Microbiome and Metabolome in Infants with Cow's Milk Protein Allergy.International journal of molecular sciences · 2023Trial
- Effects of Infant Formula With Human Milk Oligosaccharides on Growth and Morbidity: A Randomized Multicenter Trial.Journal of pediatric gastroenterology and nutrition · 2017Trial
- Anti-Pneumococcal Properties of the Native Human Milk Oligosaccharide Fraction: A Concentration-Dependent Study.International journal of molecular sciences · 2025Article
- Challenges and Opportunities in Quantifying Bioactive Compounds in Human Breastmilk.Biomolecules · 2025Review
- Absolute quantification of eight human milk oligosaccharides in breast milk to evaluate their concentration profiles and associations with infants' neurodevelopmental outcomes.Journal of food science · 2024Article
- Analysis of human biological samples using porous graphitic carbon columns and liquid chromatography-mass spectrometry: a review.Analytical and bioanalytical chemistry · 2024Review
- Human breast tissue engineering in health and disease.EMBO molecular medicine · 2024Review
- [Applications of chromatography in glycomics].Se pu = Chinese journal of chromatography · 2024Review
- Article
- Real-world evidence study on tolerance and growth in infants fed an infant formula with two human milk oligosaccharides vs mixed fed and exclusively breastfed infants.Molecular and cellular pediatrics · 2023Article
- Prebiotics as a Tool for the Prevention and Treatment of Obesity and Diabetes: Classification and Ability to Modulate the Gut Microbiota.International journal of molecular sciences · 2022Review
- Structural characterization of human milk oligosaccharides using ultrahigh performance liquid chromatography-helium charge transfer dissociation mass spectrometry.Glycobiology · 2022Article
- Determining the metabolic fate of human milk oligosaccharides: it may just be more complex than you think?Gut microbiome (Cambridge, England) · 2022Review
- Infant Formula With a Specific Blend of Five Human Milk Oligosaccharides Drives the Gut Microbiota Development and Improves Gut Maturation Markers: A Randomized Controlled Trial.Frontiers in nutrition · 2022Article
- Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-Frontiers in chemistry · 2022Article
- The Mean of Milk: A Review of Human Milk Oligosaccharide Concentrations throughout Lactation.Nutrients · 2021Review
- Changes in HMO Concentrations throughout Lactation: Influencing Factors, Health Effects and Opportunities.Nutrients · 2021Review
- Combining HPAEC-PAD, PGC-LC-MS, and 1DJournal of agricultural and food chemistry · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Defining the biological roles of human milk oligosaccharides (HMOS) requires an efficient, simple, reliable, and robust analytical method for simultaneous quantification of oligosaccharide profiles from multiple samples. The HMOS fraction of milk is a complex mixture of polar, highly branched, isomeric structures that contain no intrinsic facile chromophore, making their resolution and quantification challenging. A liquid chromatography-mass spectrometry (LC-MS) method was devised to resolve and quantify 11 major neutral oligosaccharides of human milk simultaneously. Crude HMOS fractions are reduced, resolved by porous graphitic carbon high-performance liquid chromatography (HPLC) with a water/acetonitrile gradient, detected by mass spectrometric specific ion monitoring, and quantified. The HPLC separates isomers of identical molecular weights, allowing 11 peaks to be fully resolved and quantified by monitoring mass-to-charge (m/z) ratios of the deprotonated negative ions. The standard curves for each of the 11 oligosaccharides is linear from 0.078 or 0.156 to 20 μg/ml (R(2)>0.998). Precision (coefficient of variation) ranges from 1% to 9%. Accuracy is from 86% to 104%. This analytical technique provides sensitive, precise, and accurate quantification for each of the 11 milk oligosaccharides and allows measurement of differences in milk oligosaccharide patterns between individuals and at different stages of lactation.
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