ArticleMolecular microbiology2012
A role for topoisomerase III in Escherichia coli chromosome segregation.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed.
- Topoisomerase III limits RecA-dependent DNA amplification in the chromosome terminus with RecG.Nucleic acids research · 2026Article
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- TOP3A coupling with replication forks and repair of TOP3A cleavage complexes.Cell cycle (Georgetown, Tex.) · 2024Review
- Characterization of a pathway of genomic instability induced by R-loops and its regulation by topoisomerases in E. coli.PLoS genetics · 2023Article
- Variable DNA topology is an epigenetic generator of physiological heterogeneity in bacterial populations.Molecular microbiology · 2023Review
- Negative supercoils regulate meiotic crossover patterns in budding yeast.Nucleic acids research · 2022Article
- The regulation of DNA supercoiling across evolution.Protein science : a publication of the Protein Society · 2021Review
- Lytic bacteriophages facilitate antibiotic sensitization ofAntimicrobial agents and chemotherapy · 2021Article
- The many lives of type IA topoisomerases.The Journal of biological chemistry · 2020Review
- Supercoiling, R-loops, Replication and the Functions of Bacterial Type 1A Topoisomerases.Genes · 2020Review
- Topoisomerase III Acts at the Replication Fork To Remove Precatenanes.Journal of bacteriology · 2019Article
- Direct observation of topoisomerase IA gate dynamics.Nature structural & molecular biology · 2018Article
- Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.Molecular microbiology · 2018Article
- Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli.PLoS genetics · 2018Article
- The Macromolecular Machines that Duplicate the Escherichia coli Chromosome as Targets for Drug Discovery.Antibiotics (Basel, Switzerland) · 2018Review
- The interplay between DNA topology and accessory factors in site-specific recombination in bacteria and their bacteriophages.Science progress · 2016Review
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
- MatP regulates the coordinated action of topoisomerase IV and MukBEF in chromosome segregation.Nature communications · 2016Article
- Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation.Nucleic acids research · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
Abstract
The cellular function of Escherichia coli topoisomerase III remains elusive. We show that rescue of temperature-sensitive mutants in parE and parC (encoding the subunits of the chromosomal decatenase topoisomerase IV) at restrictive temperatures by high-copy suppressors is strictly dependent on topB (encoding topoisomerase III). Double mutants of parEΔtopB and parCΔtopB were barely viable, grew slowly, and were defective in chromosome segregation at permissive temperatures. The topB mutant phenotype did not result from accumulation of toxic recombination intermediates, because it was not relieved by mutations in either recQ or recA. In addition, in an otherwise wild-type genetic background, ΔtopB cells treated with the type II topoisomerase inhibitor novobiocin displayed aberrant chromosome segregation. This novobiocin sensitivity was attributable to an increased demand for topoisomerase IV and is unlikely to define a new role for topoisomerase III; therefore, these results suggest that topoisomerase III participates in orderly and efficient chromosome segregation in E. coli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.