ArticleRespiratory research2012
Heterogeneity of respiratory dendritic cell subsets and lymphocyte populations in inbred mouse strains.
Article in Respiratory research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
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- Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.Laboratory investigation; a journal of technical methods and pathology · 2019Article
- Peptidoglycan Recognition Protein 2 Regulates Neutrophil Recruitment Into the Lungs AfterFrontiers in microbiology · 2019Article
- Peptidoglycan Recognition Protein 3 Does Not Alter the Outcome of Pneumococcal Pneumonia in Mice.Frontiers in microbiology · 2018Article
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- Origin, Localization, and Immunoregulatory Properties of Pulmonary Phagocytes in Allergic Asthma.Frontiers in immunology · 2016Review
- Prospectively defined murine mesenchymal stem cells inhibit Klebsiella pneumoniae-induced acute lung injury and improve pneumonia survival.Respiratory research · 2015Article
- Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model.Journal of visualized experiments : JoVE · 2015Article
- Acute adaptive immune response correlates with late radiation-induced pulmonary fibrosis in mice.Radiation oncology (London, England) · 2015Article
- Caspase-1-dependent and -independent cell death pathways in Burkholderia pseudomallei infection of macrophages.PLoS pathogens · 2014Article
- Pro-inflammatory chemokine CCL2 (MCP-1) promotes healing in diabetic wounds by restoring the macrophage response.PloS one · 2014Article
- Flow cytometric analysis of macrophages and dendritic cell subsets in the mouse lung.American journal of respiratory cell and molecular biology · 2013Article
- Modulation of respiratory dendritic cells during Klebsiella pneumonia infection.Respiratory research · 2013Article
- Skin TLR7 triggering promotes accumulation of respiratory dendritic cells and natural killer cells.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInbred mouse strains are used in different models of respiratory diseases but the variation of critical respiratory leukocyte subpopulations across different strains is unknown.
methodsBy using multiparameter flow cytometry we have quantitated respiratory leukocyte subsets including dendritic cells subpopulations, macrophages, classical T and B cells, natural killer cells, γδTCR+ T cells and lineage-negative leukocytes in the five most common inbred mouse strains BALB/c, C57BL/6, DBA/2, 129SV and C3H. To minimize confounding environmental factors, age-matched animals were received from the same provider and were housed under identical specific-pathogen-free conditions.
resultsResults revealed significant strain differences with respect to respiratory neutrophils (p=0.005; up to 1.4 fold differences versus C57BL/6 mice), eosinophils (p=0.029; up to 2.7 fold), certain dendritic cell subsets (p≤0.0003; up to 3.4 fold), T (p<0.001; up to 1.6 fold) and B lymphocyte subsets (p=0.005; up to 0.4 fold), γδ T lymphocytes (p=0.003; up to 1.6 fold), natural killer cells (p<0.0001; up to 0.6 fold) and lineage-negative innate leukocytes (p≤0.007; up to 3.6 fold). In contrast, total respiratory leukocytes, macrophages, total dendritic cells and bronchoalveolar lavage leukocytes did not differ significantly. Stimulation of respiratory leukocytes via Toll-like receptor 4 and 9 as well as CD3/CD28 revealed significant strain differences of TNF-α and IL-10 production.
conclusionOur study demonstrates significant strain heterogeneity of respiratory leukocyte subsets that may impact respiratory immunity in different disease models. Additionally, the results may help identification of optimal strains for purification of rare respiratory leukocyte subsets for ex vivo analyses.
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