ArticleHelicobacter2012
Antibodies anti-CagA cross-react with trophoblast cells: a risk factor for pre-eclampsia?
Article in Helicobacter, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- A Comprehensive Survey of the Relationship betweenArchives of Iranian medicine · 2022Pooled it
- Systematic Review of Mendelian Randomization Studies onInterdisciplinary perspectives on infectious diseases · 2025Review
- Assessment of adverse pregnancy outcomes associated with Helicobacter pylori infection.Scientific reports · 2024Article
- The association of Helicobacter pylori with adverse pregnancy outcomes in three European birth cohorts.BMC pregnancy and childbirth · 2024Article
- Preeclampsia in the Context of COVID-19: Mechanisms, Pathophysiology, and Clinical Outcomes.American journal of reproductive immunology (New York, N.Y. : 1989) · 2024Review
- Causal associations betweenFrontiers in cellular and infection microbiology · 2024Article
- Association of Serum Hepcidin With Preeclampsia: A Systematic Review and Meta-Analysis.Cureus · 2022Review
- Evaluating the relationship betweenAnnals of medicine and surgery (2012) · 2021Article
- The effects ofAnnals of translational medicine · 2021Article
- Relationship BetweenInternational journal of general medicine · 2020Article
- Helicobacter pylori infection selectively increases the risk for carotid atherosclerosis in young males.Atherosclerosis · 2019Article
- Human infectious diseases and risk of preeclampsia: an updated review of the literature.Infection · 2017Review
- Helicobacter pylori infection: a predictor of vomiting severity in pregnancy and adverse birth outcome.American journal of obstetrics and gynecology · 2017Article
- Helicobacter pylori colonization and pregnancies complicated by preeclampsia, spontaneous prematurity, and small for gestational age birth.Helicobacter · 2017Article
- Effect of maternal Helicobacter Pylori infection on birth weight in an urban community in Uganda.BMC pregnancy and childbirth · 2016Article
- A Dormant Microbial Component in the Development of Preeclampsia.Frontiers in medicine · 2016Review
- Barriers and Challenges in Hyperemesis Gravidarum Research.Nutrition and metabolic insights · 2015Review
- Beyond the stomach: an updated view of Helicobacter pylori pathogenesis, diagnosis, and treatment.World journal of gastroenterology · 2014Review
- Can Helicobacter pylori infection influence human reproduction?World journal of gastroenterology · 2014Review
- Helicobacter pylori and pregnancy-related disorders.World journal of gastroenterology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundPrevious studies reported an epidemiological association between CagA-positive H. pylori strains and pre-eclampsia. As antibodies anti-CagA cross-react with endothelial cells and trophoblast cells show an endothelial phenotypic profile, we hypothesized that anti-CagA antibodies may recognize antigens of cytotrophoblast cells, thus impairing their function. MATERIALS AND
methodsPlacenta samples were obtained from healthy women. Cytotrophoblast cells were cultured in a medium containing increasing concentration of polyclonal anti-CagA antibodies. Binding of anti-CagA antibodies to cytotrophoblast cells was evaluated by cell ELISA and immunofluorescence assay. Invasive potential of those cells was assessed by an invasion culture system and by measuring of MMP-2. Protein sequencing was performed on antigens precipitated by anti-CagA antibodies. Measurement of phosphorylated ERK expression and NF-kB DNA-binding activity in trophoblast cells incubated with anti-CagA or irrelevant antibodies was also performed.
resultsAnti-CagA antibodies recognized β-actin of cytotrophoblast cells, showing a dose-dependent binding. Incubation of cytotrophoblast cells with increasing doses of anti-CagA antibodies significantly reduced their invasiveness and determined a significant decrease in phosphorylated ERK expression and a reduced NF-kB translocation activity.
conclusionsThis study shows that anti-CagA antibodies recognize β-actin of cytotrophoblast cells, reducing their invasiveness ability, possibly giving a biological explanation for the epidemiological association.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.