Effect of phosphatidylcholine on pld gene expression level of Aspergillus fumigatus by the real time PCR method and investigations of these genes using bioinformatics analysis.

Ad Noodeh, N Singh, Gd Robson

Open access · greenAbstract read

In one paragraph

Article in Iranian journal of microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 46% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Ad NoodehDepartment of Life Sciences, Faculty of Science and Technology, Anglia Ruskin University, Cambridge, UK.

N Singh

Gd Robson

Anglia Ruskin University · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesPhospholipases are a group of enzymes that breakdown phospholipid molecules producing second products. These second products play a diverse role in the cell such as signal transduction and digestion in humans. In this study, the effect of phospholipids on the expression of pld genes of A. fumigatus was investigated. The pld genes of this fungus were also investigated using bioinformatics studies. MATERIALS AND

methodsReal-time PCR was performed to study the expression of pld genes. These genes were investigated using bioinformatics studies.

resultsThere was more significant expression for all three pld genes when A. fumigatus was grown in the presence of phospholipids in the medium. The sequence of pld genes of A. fumigatus was also interrogated using bioinformatics analysis and their relationship with the other microorganisms was investigated. The fungal pld genes were more closely related to pld genes from animals and least related to bacterial pld genes.

conclusionafpld1, afpld2 and afpld3 are expressed and are up-regulated by phosphatidylcholine. Although indirect evidence of extracellular PLD activity in A. fumigatus was demonstrated, conclusive proof by partially sequencing the isolated protein will be needed and its significance in pathogenicity will have to be assessed by constructing a knockout strain and testing its virulence in a mouse model.

Indexed as

Aspergillus fumigatusgene expressionphospholipase D (PLD)phospholipidpld genereal time PCRrelationship tree

Identifiers

PMID23066489

PMCPMC3465540

OpenAlexW2166353283

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.