ArticleNucleic acids research2012
DNA catenation maintains structure of human metaphase chromosomes.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Topoisomerase II Inhibition in Cancer: A Focus on Metal Complexes.Mini reviews in medicinal chemistry · 2025Review
- Cell cycle responses to Topoisomerase II inhibition: Molecular mechanisms and clinical implications.The Journal of cell biology · 2023Review
- Defining a core configuration for human centromeres during mitosis.Nature communications · 2023Article
- Telling Your Right Hand from Your Left: The Effects of DNA Supercoil Handedness on the Actions of Type II Topoisomerases.International journal of molecular sciences · 2023Review
- Defining a core configuration for human centromeres during mitosis.bioRxiv : the preprint server for biology · 2023Article
- DNA-Stimulated Liquid-Liquid phase separation by eukaryotic topoisomerase ii modulates catalytic function.eLife · 2022Article
- Recognition of DNA Supercoil Handedness during Catenation Catalyzed by Type II Topoisomerases.Biochemistry · 2022Article
- Control of topoisomerase II activity and chemotherapeutic inhibition by TCA cycle metabolites.Cell chemical biology · 2022Article
- PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes.Molecular biology of the cell · 2020Article
- Review
- Superresolution microscopy reveals linkages between ribosomal DNA on heterologous chromosomes.The Journal of cell biology · 2019Article
- A microfluidic device for isolating intact chromosomes from single mammalian cells and probing their folding stability by controlling solution conditions.Scientific reports · 2018Article
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- Novel insights into mitotic chromosome condensation.F1000Research · 2016Review
- TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.Nature communications · 2015Article
- DNA Topoisomerases.EcoSal Plus · 2015Review
- Mitotic catenation is monitored and resolved by a PKCε-regulated pathway.Nature communications · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Mitotic chromosome structure is pivotal to cell division but difficult to observe in fine detail using conventional methods. DNA catenation has been implicated in both sister chromatid cohesion and chromosome condensation, but has never been observed directly. We have used a lab-on-a-chip microfluidic device and fluorescence microscopy, coupled with a simple image analysis pipeline, to digest chromosomal proteins and examine the structure of the remaining DNA, which maintains the canonical 'X' shape. By directly staining DNA, we observe that DNA catenation between sister chromatids (separated by fluid flow) is composed of distinct fibres of DNA concentrated at the centromeres. Disrupting the catenation of the chromosomes with Topoisomerase IIα significantly alters overall chromosome shape, suggesting that DNA catenation must be simultaneously maintained for correct chromosome condensation, and destroyed to complete sister chromatid disjunction. In addition to demonstrating the value of microfluidics as a tool for examining chromosome structure, these results lend support to certain models of DNA catenation organization and regulation: in particular, we conclude from our observation of centromere-concentrated catenation that spindle forces could play a driving role in decatenation and that Topoisomerase IIα is differentially regulated at the centromeres, perhaps in conjunction with cohesin.
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Registered trials
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