ArticleJournal of bacteriology2012
Metal resistance and lithoautotrophy in the extreme thermoacidophile Metallosphaera sedula.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula.Extremophiles : life under extreme conditions · 2024Article
- Improved protocol for metabolite extraction and identification of respiratory quinones in extremophilic Archaea grown on mineral materials.Frontiers in microbiology · 2024Article
- "Freezing" Thermophiles: From One Temperature Extreme to Another.Microorganisms · 2022Review
- Microbial adaptation to different environmental conditions: molecular perspective of evolved genetic and cellular systems.Archives of microbiology · 2022Review
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Extremophiles, a Nifty Tool to Face Environmental Pollution: From Exploitation of Metabolism to Genome Engineering.International journal of environmental research and public health · 2021Review
- Stress-tolerant non-conventional microbes enable next-generation chemical biosynthesis.Nature chemical biology · 2020Review
- Participation of UV-regulated Genes in the Response to Helix-distorting DNA Damage in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius.Microbes and environments · 2019Article
- Exploring the microbial biotransformation of extraterrestrial material on nanometer scale.Scientific reports · 2019Article
- Nanoscale Tungsten-Microbial Interface of the Metal Immobilizing Thermoacidophilic ArchaeonFrontiers in microbiology · 2019Article
- Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018Review
- In a quest for engineering acidophiles for biomining applications: challenges and opportunities.Genes · 2018Review
- Inorganic Polyphosphate, Exopolyphosphatase, andArchaea (Vancouver, B.C.) · 2018Article
- Microhomology-Mediated High-Throughput Gene Inactivation Strategy for the Hyperthermophilic Crenarchaeon Sulfolobus islandicus.Applied and environmental microbiology · 2018Article
- Evolution of copper arsenate resistance for enhanced enargite bioleaching using the extreme thermoacidophile Metallosphaera sedula.Journal of industrial microbiology & biotechnology · 2017Article
- Microbial copper resistance: importance in biohydrometallurgy.Microbial biotechnology · 2017Review
- Exploring Fingerprints of the Extreme ThermoacidophileFrontiers in microbiology · 2017Article
- Reaction kinetic analysis of the 3-hydroxypropionate/4-hydroxybutyrate COMetabolic engineering · 2016Article
- Transcriptomes of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Exposed to Metal "Shock" Reveal Generic and Specific Metal Responses.Applied and environmental microbiology · 2016Article
- Complete Genome Sequences of Evolved Arsenate-Resistant Metallosphaera sedula Strains.Genome announcements · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Archaea such as Metallosphaera sedula are thermophilic lithoautotrophs that occupy unusually acidic and metal-rich environments. These traits are thought to underlie their industrial importance for bioleaching of base and precious metals. In this study, a genetic approach was taken to investigate the specific relationship between metal resistance and lithoautotrophy during biotransformation of the primary copper ore, chalcopyrite (CuFeS(2)). In this study, a genetic system was developed for M. sedula to investigate parameters that limit bioleaching of chalcopyrite. The functional role of the M. sedula copRTA operon was demonstrated by cross-species complementation of a copper-sensitive Sulfolobus solfataricus copR mutant. Inactivation of the gene encoding the M. sedula copper efflux protein, copA, using targeted recombination compromised metal resistance and eliminated chalcopyrite bioleaching. In contrast, a spontaneous M. sedula mutant (CuR1) with elevated metal resistance transformed chalcopyrite at an accelerated rate without affecting chemoheterotrophic growth. Proteomic analysis of CuR1 identified pleiotropic changes, including altered abundance of transport proteins having AAA-ATPase motifs. Addition of the insoluble carbonate mineral witherite (BaCO(3)) further stimulated chalcopyrite lithotrophy, indicating that carbon was a limiting factor. Since both mineral types were actively colonized, enhanced metal leaching may arise from the cooperative exchange of energy and carbon between surface-adhered populations. Genetic approaches provide a new means of improving the efficiency of metal bioleaching by enhancing the mechanistic understanding of thermophilic lithoautotrophy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.