ArticleJournal of bacteriology2012
An archaeal histone is required for transformation of Thermococcus kodakarensis.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- The Thermococcales as a model system: historical perspectives and emerging tools.Journal of bacteriology · 2025Review
- Histones and histone variant families in prokaryotes.Nature communications · 2024Article
- Tetraether archaeal lipids promote long-term survival in extreme conditions.Molecular microbiology · 2024Article
- Archaeal histone-based chromatin structures regulate transcription elongation rates.Communications biology · 2024Article
- How Do Thermophiles Organize Their Genomes?Microbes and environments · 2024Review
- DNA-bridging by an archaeal histone variant via a unique tetramerisation interface.Communications biology · 2023Article
- Histones direct site-specific CRISPR spacer acquisition in model archaeon.Nature microbiology · 2023Article
- Article
- Growth temperature and chromatinization in archaea.Nature microbiology · 2022Article
- Deep Conservation of Histone Variants in Thermococcales Archaea.Genome biology and evolution · 2022Article
- An archaeal histone-like protein regulates gene expression in response to salt stress.Nucleic acids research · 2021Article
- Archaea: The Final Frontier of Chromatin.Journal of molecular biology · 2021Review
- Archaeal chromatin 'slinkies' are inherently dynamic complexes with deflected DNA wrapping pathways.eLife · 2021Article
- Extended Archaeal Histone-Based Chromatin Structure Regulates Global Gene Expression inFrontiers in microbiology · 2021Article
- Thermococcus kodakarensis provides a versatile hyperthermophilic archaeal platform for protein expression.Methods in enzymology · 2021Article
- Histone variants in archaea and the evolution of combinatorial chromatin complexity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea.Journal of bacteriology · 2020Article
- Different Proteins Mediate Step-Wise Chromosome Architectures inFrontiers in microbiology · 2020Article
- Chromatinization ofeLife · 2019Article
- The Role of Archaeal Chromatin in Transcription.Journal of molecular biology · 2019Review
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6 authors.
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Abstract
Archaeal histones wrap DNA into complexes, designated archaeal nucleosomes, that resemble the tetrasome core of a eukaryotic nucleosome. Therefore, all DNA interactions in vivo in Thermococcus kodakarensis, the most genetically versatile model species for archaeal research, must occur in the context of a histone-bound genome. Here we report the construction and properties of T. kodakarensis strains that have TK1413 or TK2289 deleted, the genes that encode HTkA and HTkB, respectively, the two archaeal histones present in this archaeon. All attempts to generate a strain with both TK1413 and TK2289 deleted were unsuccessful, arguing that a histone-mediated event(s) in T. kodakarensis is essential. The HTkA and HTkB amino acid sequences are 84% identical (56 of 67 residues) and 94% similar (63 of 67 residues), but despite this homology and their apparent redundancy in terms of supporting viability, the absence of HTkA and HTkB resulted in differences in growth and in quantitative and qualitative differences in genome transcription. A most surprising result was that the deletion of TK1413 (ΔhtkA) resulted in a T. kodakarensis strain that was no longer amenable to transformation, whereas the deletion of TK2289 (ΔhtkB) had no detrimental effects on transformation. Potential roles for the archaeal histones in regulating gene expression and for HTkA in DNA uptake and recombination are discussed.
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